降解五氯酚的微好氧颗粒污泥的培养及其微生物种群分析  

Cultivation of Micro-aerobic Granules and Analysis of Their Microbial Populations for Pentachlorophenol Degradation

作　　者：陈竹[1] 陈元彩[1] 蓝惠霞[2] 付时雨[1] 詹怀宇[1] 叶豪[1] 黄堰[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广州510640 [2]青岛科技大学环境与安全工程学院,青岛266042

出　　处：《应用与环境生物学报》2008年第3期416-421,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.20676045);华南理工大学制浆造纸工程国家重点实验室开放基金项目(No.200552);学生研究工作计划的部分研究内容~~

摘　　要：在微好氧的条件下培养出颗粒污泥并用含五氯酚(PCP)的废水对颗粒污泥进行驯化,研究了培养过程中颗粒污泥的MLSS、SVI、粒径以及对COD和PCP处理能力的变化.颗粒污泥培养成熟后,PCP和COD的去除率分别达到85.3%和86.1%.用扫描电镜观察了颗粒污泥的结构,颗粒污泥内细菌种群丰富,大多数为短杆菌和球菌.同时采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术对培养出的微好氧颗粒污泥以及用PCP驯化后的颗粒污泥中微生物的种群进行了初步分析和对比,用PCP驯化后的微生物种群发生了较明显的动态变化,新增了某些菌群,如不动杆菌(Acinetobacter)、变形杆菌(Proteobacterium)和硫酸盐还原菌等.Micro-aerobic granules were considered as an effective method to achieve complete degradation of pentachlorophenol （PCP）, because they made anaerobic and aerobic environments occur simultaneously. The microbial populations in granules were very important for understanding the principle of PCP degradation. Granular sludge was cultivated under micro-aerobic condition, and the micro-aerobic granules were domesticated using wastewater which consisted of PCP, The variations in MLSS, SVI, PN/PS and the size of sludge were detected during cultivation. In the cultivation, the MLSS and PN/PS increased, while the SVI decreased gradually. After the granular sludge was matured, the removal rates of PCP and COD reached 85.3% and 86. 1%, respectively. The observations on the matured granules were done by scanning electron microscope, which showed that the microbes were abundant in the granules and most of them were short bacili and cocci. Simultaneously, the microbial community structure of the granules cultivated with and without PCP was analyzed, and was compared using DGGE. Compared to that without PCP, the microbial community of the granules with PCP was found with some new microbes, such as Acinetobacter, Proteobacterium and sulfate-reducing bacteria.

关 键 词：微好氧 颗粒污泥 五氯酚 微生物种群 PCR—DGGE 

分 类 号：X703[环境科学与工程—环境工程] X172