微波诱变选育耐酸高效厌氧产氢菌  

Screening of Highly Efficient H_2-producing Aciduric Anaerobic Strain Using Microwave Mutagenesis

作　　者：宋丽[1] 刘晓风[1] 刘培旺[1] 袁月祥[1] 闫志英[1] 何荣玉[1] 贺蓉娜[1] 廖银章[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2008年第3期427-431,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑计划(No.2006BAD07A02)资助~~

摘　　要：为获得厌氧产氢菌的高效突变株,以产氢菌H-8为原始菌株进行微波诱变处理,对微波诱变参数进行了优化,考察了突变株的遗传稳定性、产氢特性及耐酸性.经过微波(低火)物理诱变得到5株高产氢突变株HW7、HW33、HW181、HW184和HW195,多次传代实验表明,HW195是稳定的高产突变株.突变株HW195具有较好的耐酸性,在pH值为2·8时仍能生长.通过间歇发酵实验,其最大产氢量和最大产氢速率分别达到2460mL/L培养基和340mLL-1h-1,比原始菌分别提高了50·7%和41·7%.In order to obtain highly efficient H2-producing aciduric anaerobic strain, a hydrogen producing strain H-8 was irradiated by microwave, the microwave mutagenesis parameters were optimized, and the genetic stability, hydrogen-producing potential and acid resistance of the mutant were investigated. An aciduric mutant with steady hydrogen-producing capability was obtained and it could grow at pH 2. 8. Its capability of hydrogen production was measured in the batch culture experiment. The maximum hydrogen yield was 2 460 mL/L and hydrogen production rate 340 mL· L^-1· h^-1 , and they were 50. 7% and 41.7% higher than those of the initial strain, respectively. The results demonstrated that microwave mutagenesis could be used for hydrogen producing microorganisms.

关 键 词：生物制氢 厌氧 产氢菌 微波诱变 耐酸 

分 类 号：Q93[生物学—微生物学]