海洋细菌Bacillus sp.次生代谢产物的研究  

The metabolites from the broth of marine bacterium Bacillus sp.

作　　者：乔莉[1] 周玉枝[1] 陈欢[1] 姚遥[1] 徐剑锟[1] 裴月湖[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国药物化学杂志》2008年第3期219-221,共3页Chinese Journal of Medicinal Chemistry

基　　金：国家高技术研究发展计划项目(2007AA09Z413)

摘　　要：目的对海洋细菌Bacillussp.发酵液的乙酸乙酯萃取物进行化学成分的研究。方法采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等手段,利用理化和波谱分析方法,对海洋细菌Bacillussp.发酵液的乙酸乙酯萃取物的化学成分进行分离鉴定。结果从该细菌发酵液的乙酸乙酯萃取物中分离得到9个化合物,经光谱数据分析,鉴定它们的结构分别为苯丙氨酸(1)、对羟基苯乙胺(2)、3-另丁基-吡咯并哌嗪-1,4-二酮(3)、3-异丁基-吡咯并哌嗪-1,4-二酮(4)、3-苯甲基-7-羟基-吡咯并哌嗪-1,4-二酮(5)、3-苯甲基-吡咯并哌-嗪1,4-二酮(6)、3-异丙基-吡咯并哌嗪-1,4-二酮(7)、胸腺嘧啶(8)、尿嘧啶(9)。结论9个化合物均是首次从该细菌代谢产物中分离得到。Aim To study the chemical constituents from the broth of marine bacterium Bacillus sp. Methods The compounds were separated by column chromatography and their structures were elucidated based on chemical and spectral analysis. Results Nine compounds were isolated from the acetate extracts of the broth of Bacillus sp. Their structures were determined as phenylalanine（1）, p-hydroxy-phenylethylamine（2）, 3- sec-butyl-pyrrolopiperazine-1, 4-dione （ 3 ）, 3-isobutyl-pyrrolopiperazine-1, 4-dione （4）, 3-benzyl-7-hydroxy-pyrrolopiperazine-1,4-dione （ 5 ）, 3-benzyl-pyrrolopiperazine-1, 4-dione （ 6 ）, 3-isopropyl-pyrrolopiperazine-1, 4-dione（7）, thymine（8）, and uracil（9）. Conclusion All of the compounds are obtained from the metabolites of marine bacterium Bacillus sp. for the first time.

关 键 词：结构鉴定 柱色谱法 海洋细菌 乙酸乙酯萃取物 芽胞杆菌 

分 类 号：R284[医药卫生—中药学]