铕苯丙氨酸配合物与DNA相互作用的循环伏安法和紫外光谱法研究  

Study on Interaction between Eu Phenylanaline and DNA by Using of Cyclic Voltammetry and UV Spectroscopy

作　　者：沈鹤柏[1] 蒯丽衡[1] 倪丽华[1] 李建粤 章宗穰[1] 

机构地区：[1]上海师范大学化学系

出　　处：《中国稀土学报》1997年第2期182-185,共4页Journal of the Chinese Society of Rare Earths

基　　金：上海市教委重点学科基金

摘　　要：运用循环伏安法和紫外光谱法研究了Ｅｕ（ｐｈｅ）３＋３与ＤＮＡ的相互作用。Ｅｕ（ｐｈｅ）３＋３与ＤＮＡ作用后，循环伏安曲线的峰电流密度减小，峰电位差稍有增加，扩散系数减小。紫外光谱的特征吸收峰呈明显的减色效应。这可能是Ｅｕ（ｐｈｅ）３＋３与ＤＮＡ因静电引力形成加合物以及Ｅｕ（ｐｈｅ）３＋３有很强的遮蔽负电荷的作用，促使碱基面与碱基面的进一步堆积（ｓｔａｃｋｉｎｇ）所致。The interaction between DNA and the Eu(phe) 3+ 3 complex ion was studied by using of cyclic voltammetry and differential UV spectroscopy. The results depicted an obvious decrease of peak current in CV plot after the interaction between two studied species. The observed peak potential separation was increased, but the diffusion coefficient of Eu complex ion was decreased. Hypochromicity was observed at 226 nm and 258 nm after the predicted interaction of them. Preliminary interpretation is proposed for discussion.

关 键 词：小牛胸腺 循环伏安法 紫外光谱 铕苯丙氨酸 配位 

分 类 号：O657.14[理学—分析化学] O657.32[理学—化学]