重组瘦素诱导HSC增殖及保肝宁调控作用的实验研究  

Experimental study on the proliferation of HSC indcued by recombinant leptin and the regulation and control action of baoganning

作　　者：侯丽颖[1] 季幸姝[1] 吕志平[2] 贺松其[2] 文彬 蔡红兵[2] 

机构地区：[1]广州中医药大学,广东广州510400 [2]南方医科大学中医药学院,广东广州510515 [3]解放军458医院,广东广州510602

出　　处：《现代医药卫生》2008年第13期1897-1898,共2页Journal of Modern Medicine & Health

基　　金：国家自然基金资助课题(编号:30672769);广东省中医药局基金资助课题(编号1050128)

摘　　要：目的:探讨中药复方保肝宁对外源性瘦素(leptin)诱导的肝星状细胞(Hepatic stellate cell,HSC)增殖功能的影响及其保肝宁调控作用的可能机制。方法:给正常Wistar大鼠灌胃保肝宁煎剂7天,制备含药血清,用甲基噻唑基四唑(methyl thiazolylium,MTT)检测保肝宁对leptin刺激HSC-LX2增殖的影响;应用蛋白印迹实验(Western blotting analysis)检测的P-JAK2蛋白表达水平。结果:保肝宁组作用HSC-LX2 6小时后,P-JAK2蛋白的表达水平开始降低,P-JAK2处于最低水平。与正常血清组比较,保肝宁组较秋水仙碱组更能降低P-JAK2蛋白表达水平。结论:中药复方保肝宁有阻断leptin诱导HSCs-LX2增殖的作用,而抑制P-JAK2蛋白的表达水平可能是其主要的机制之一。Objective：To investigate the effect of the compound prescription named as baoganning made of chinese traditional medicine on proliferation of hepatic steUate cells （HSCs） actived by leptin,we try to clarify the mechanisms that baoganning resisted haptic fibrosis.Methods：Normal wistar rats were medicated with baoganning decoction by gavage for 7 days to prepare baoganning drugserum. The effect of baoganning drug-serum on 100 ng/ml leptin activing HSC-LX2 growth was measured by MTT assay;P-JAK2 at different time after baoganning stimulation was detected by Western blotting analysis.Results：Compared to the blank control,P-JAK2 began to descend at 6h after the serum with baoganning ingredients treating HSC-LX2 and JAK2 reached to the lowest level at 12h. Compared to the blank control,baoganning ingredients treating HSC-LX2 which was excelled compared with eolchicine ingredients.Conclusion ：Baoganning could inhibit P-JAK2, and the one of the mechanisms baoganning preventing Hepatic fibrosis may be because inhibiting P-JAK2.

关 键 词：保肝宁 瘦素 肝星状细胞 P-JAK2 

分 类 号：R28[医药卫生—中药学]