γ射线对斜卧青霉的诱变筛选及产酶条件优化  

MUTANT STRAIN SCREENING BY ^(60)Co γ-RAYS IRRADIATION AND ITS CELLULASE ENZYME PRODUCE CONDITION

作　　者：宋安东[1] 苏丽娟 谢慧[1] 曲音波[3] 杨铭[4] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]河南天冠集团博士后科研工作站,河南南阳473000 [3]山东大学生命科学学院博士后流动站,山东济南250100 [4]商丘职业技术学院农学系,河南商丘476000

出　　处：《核农学报》2008年第3期280-285,共6页Journal of Nuclear Agricultural Sciences

基　　金：河南省杰出人才创新基金项目(321001300);国家农业成果转化资金项目(2006GB2D000173)

摘　　要：以斜卧青霉(Penicillium decumbens)A10为出发菌株,经450 Gy60Coγ射线诱变处理,选育出1株纤维素酶高产菌株A50,对其产纤维素酶的液态发酵条件进行研究优化,确定了所试因素的最佳组合,即主碳源浓度为5%,其中麸皮与玉米秸秆比例为1∶1,辅加碳源为0.1%的葡萄糖,辅加氮源为0.2%的磷酸氢二铵,Tween-80添加量为0.1%,培养基初始pH为5.0,300ml三角瓶的装液量为30ml、接种量10%、培养温度为32℃、摇床转速200r/min。发酵至60h时,纤维素酶活和滤纸酶活均达到最高,分别为27.28和1.98IU/ml,较出发菌株A10分别提高了33.2%和45.59%。A mutant strain A50 with high cellulase activity was induced and isolated by using ^60 Co γ-rays irradiation from the initial Penicillium decumbens A10. The optimum fermentation conditions of A50 were investigated through orthogonal designing experiment, the major carbon resource 5 %, the ratio between wheat bran and corn straw 1 ： 1, the concentration of glucose as supplemental carbon 0.1%, the concentration of （NH4）2HPO4 as supplemental nitrogen resource 0.2%, the initial pH of liquid medium 5.0, the inoculated amount for fermentation 10% and the concentration of Tween-80 0.1%, 30ml initial media filled in the 300ml flask with culture condition of 32 ℃and 200r/min. Under the optimum conditions mentioned above, the highest activities of cellulase and filter paper enzyme were 27.28 and 1.98IU/ml at 60h fermentation, respectively, which was 33.2 % and 45.59 % higher than those of the initial strain.

关 键 词：^60 Co Γ射线辐照 斜卧青霉A10 诱变 发酵 纤维素酶 

分 类 号：S476[农业科学—农业昆虫与害虫防治]