用核素示踪研究小麦根系Na^＋外排速率的两种方法  

METHODS OF Na^+ EFFLUX RATE OF WHEAT ROOT MEASURED BY NUCLIDE TRACER

作　　者：王春梅[1] 李湛[2] 伍国强[1] 张金林[1] 王锁民[1] 

机构地区：[1]兰州大学草地农业科技学院,甘肃兰州730000 [2]兰州大学核科学与技术学院,甘肃兰州730000

出　　处：《核农学报》2008年第3期370-373,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30770347,30671488,J0630962);863计划(2006AA10Z126)

摘　　要：采用22Na+同位素示踪法研究小麦在NaCl胁迫下根系的Na+外排能力,并对植株法与外排液法2种测定方法进行了比较。结果表明,小麦根系在NaCl胁迫下具有明显的Na+外排能力,且随着NaCl浓度增加而增强,以阻止过多的盐分在体内积累。使用外排液法在3个盐浓度下所测得Na+外排速率均略高于植株法,但2种测定方法差异不显著,结果基本一致。综合比较表明,在植物根系Na+外排速率的测定中,拟推荐采用外排液法,使操作简便并能减少系统误差。Two methods of nuclide isotope （^22 Na^＋ ） tracer methods： plant analyzing and efflux solution analyzing, were used and compared for measuring Na^＋ efflux rate of wheat mot. The results showed that roots of wheat had evident ability to pump the Na^＋ out and enhanced along with the increase of NaCl stress concentration to prevent excessive Na^＋ accumulated in plant. Na^＋ efflux rates were no significant difference between the two methods, though the rates were little higher from efflux solution analyzing method. The efflux solution analyzing method was recommended due to its facility and low system error.

关 键 词：放射性同位素 ^22Na^+ 示踪技术 NA^+ 外排速率测定方法 

分 类 号：S512.1[农业科学—作物学] S124