藏药材抗新城疫病毒的体外试验研究  

Anti-NDV Effects of Tibetan Herbal Medicines in Vitro

作　　者：牛家强[1] 徐业芬[1] 王君敏[2] 胡元亮[2] 李德胜[1] 索珍[1] 

机构地区：[1]西藏大学农牧学院动物科学技术学院,西藏林芝860000 [2]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《中国兽药杂志》2008年第6期16-20,共5页Chinese Journal of Veterinary Drug

基　　金：西藏自治区科学技术厅重点项目"抗禽源性病毒藏药材筛选及制剂研制"

摘　　要：为筛选抗新城疫病毒藏药复方的组分药,将安全浓度范围内的藏菖蒲、红景天、灵芝菌柄、灵芝菌盖、决明子、天冬、龙胆、降香、乳香、仁青芒觉、二十五味松石丸和二十五味珍珠丸的煎液和鸡新城疫病毒(NDV)分别以3种方式(先加藏药后接种病毒、先接种病毒后加藏药、藏药和病毒感作后同时加)加入到鸡胚成纤维细胞(CEF)的培养体系中,72h后用MTT法测定各藏药对NDV感染CEF能力的影响。结果表明,多数藏药均能显著抑制NDV感染细胞,且与加药方式和药物浓度有一定关系。藏菖蒲、灵芝菌盖和降香在3种加药方式中均呈现显著效果,可作为抗NDV藏药复方的组分药。In order to select the component drugs from Tibetan herbal medicinal prescriptions resisting new castle disease virus （ NDV）, the decoctions of twelve Tibetan herbal medicines （ THMs）, Tibetan acorus calamus, Integripetal rhodiola herb, Ganoderma lucidum stipe, Ganoderma lucidum pileus, Cassia seed, Cochinchinese asparagus root, Gentian, Lignum acronylchiae, Libanotus, Ershiwuwei Zhenzhu Wan within cellular safety concentrations Renqingmangjue, Ershiwuwei Songshi Wan and and NDV were added into chick embryo fibroblast （CEF） in three ways （ pre -, post - and mixed - administration）. The effects of THMs on cellular infectivity of NDV were assayed by MTY method. The results showed that most of the THMs could inhibit NDV from infecting CEF significantly, which was related to the administration mode and drug concentrations. Tibetan acorus calamus, Ganoderma lucidum pileus and Lignum acronylchiae presented better effects in three adding mode and could be considered as component drugs of anti - NDV Tibetan herbal medicinal prescriptions.

关 键 词：藏药材 细胞培养 新城疫病毒 感染细胞能力 

分 类 号：S853.72[农业科学—临床兽医学]