青霉素对乳酸-丙酮-溴酸钾-硫酸锰-硫酸开放化学振荡体系的扰动  

Disturbance of Penicillin to the Lactic Acid-Acetone-KBrO_3 Mn^(2+)-H_2SO_4 Oscillating System with Analyte Pulse Perturbation Technique

作　　者：高锦章[1] 曲婕[1] 魏晓霞[1] 郭淼[1] 任杰[1] 杨武[1] 

机构地区：[1]西北师范大学化学化工学院,兰州730070

出　　处：《分析化学》2008年第6期735-739,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20475044);中国-乌克兰政府间科技合作基金(No.043-05);西北师范大学科技创新工程(No.KJCXGC-01)资助项目

摘　　要：在连续搅拌反应器(CSTR)中,利用分析物脉冲微扰技术(APP)研究了青霉素对锰离子催化Belousov-Zhabotinsky(B-Z)开放化学振荡体系的影响。结果表明,青霉素浓度在2.97×10-7～5.50×10-5mol/L范围内,周期和振幅的变化率与所加入青霉素浓度的负对数呈良好的线性关系,相关系数分别为0.9981和0.9985;检出限为5.9×10-8mol/L。所建立的方法简便易行,每小时可进样15次。对可能的反应机理也进行了讨论。锰属于生命元素,在体内参与生物酶的合成与转化,研究青霉素对锰催化振荡反应体系的扰动对于理解生命运动更为有利。A rapid and convenient method is presented for the determination of penicillin, which is based upon sequential perturbation of Mn （ II ）-catalyzed belousov-zhabotinsky （ B-Z ） oscillating system with different amounts of penicillin using a continuous-flow stirred tank reactor （CSTR）. The resuhs showed that changes both in oscillating period and amplitude were linearly proportional to the -lgC （where C is the concentration of penicillin） in either the range of 2.97×10^-7 -5.50×10^-5 mol/L or the range of 2.97×10^-6 - 5.50×10^-5 mol/L, with the regression coefficients of 0.9981 and 0.9985, respectively. A lower detection limit of 5.9×10^-8 mol/L and excellent throughput （ 15 samples/h） were obtained. The Mn（ Ⅱ ）-catalyzed oscillating system included lactic acid is more interesting for understanding the oscillator in biological system, because lactic acid （LA） is an important intermediate product in the metabolic process of human. Finally, a possible mechanism of penicillin perturbation in the oscillating reaction was also discussed.

关 键 词：化学振荡 青霉素 脉冲微扰技术 连续搅拌反应器 

分 类 号：O643.1[理学—物理化学] TP274[理学—化学]