层层自组装制备基于多壁碳纳米管的胆碱生物传感器  

Fabrication of Choline Biosensor Based on Multi-walled Carbon Nanotubes Using Layer-by-layer Assembly Technique

作　　者：秦霞[1] 王艳艳[1] 赵紫霞[1] 王新胜[1] 李莎[1] 于敏[1] 黄楠[1] 苗智颖[1] 陈强[1] 

机构地区：[1]南开大学生命科学学学院,生物活性材料教育部重点实验室,天津300071

出　　处：《分析化学》2008年第6期827-830,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30772746);天津科技发展计划基金(No.06YEGPNC03900)资助项目

摘　　要：以经混酸处理的多壁碳纳米管(MWCNTs)修饰铂(Pt)电极,在此基础上固定(PAA/PVS)3复合膜,采用层层自组装技术将高分子聚电解质PDDA与胆碱氧化酶交替组装在已修饰的电极上,构建了电流型胆碱生物传感器。实验结果表明,MWCNTs的引入使电极对H2O2的催化电流明显增大,制成的酶电极可以有效控制酶量的使用,酶膜组装层数为8时最优,对胆碱的线性响应范围为5×10-7～1×10-4mol/L;灵敏度为12.53μA/mmol;响应时间为7.60s;检出限为2×10-7mol/L(S/N=3)。传感器的抗干扰能力强,稳定性好,30d时的响应电流值仍保持最初的89.5%。3次平行实验的RSD为3.64%。A novel amperometric choline biosensor was constructed by alternately assembling poly-（ dial- lyldimethylammoium chloride） （PDDA） and choline oxidase on a platinum electrode modified by multi-walled carbon nanotubes （MWCNTs） and three layers of poly-（allylamine） （PAA）/poly-（ vinyl sulfate, potassium salt） （PVS）. The results showed that MWCNTs could improve the current response to H202 obviously, and the amount of the enzyme could be controlled well by the method. The optimum assembly number was 8. The linear range for choline concentration was 5× 10^-7 -1×10^-4mol/L with a detection limit of 2×10^-7 mol/L （S/N = 3 ）. The sensitivity was 12.53 μA/mmol with a response time of 7.60 s. The biosensor exhibited an excellent anti-interferential property, and a good stability, remaining 89. 5% of its initial response after 30 days. The RSD was 3.6%.

关 键 词：碳纳米管 聚电解质 胆碱生物传感器 层层自组装技术 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]