新鲜全血重复测定差值法在血细胞分析质量控制中的应用  

An application of repetitive measurement difference value with patient fresh whole blood for the quality control of hematocytes analysis

作　　者：曾建明[1] 李竟 陈茶[1] 徐建华[1] 庞鑫[1] 王美兰[1] 罗燕玲[1] 张秀明 

机构地区：[1]广东省中医院检验科,510120 [2]蚌埠医学院检验系,233003

出　　处：《实验与检验医学》2008年第3期224-226,共3页Experimental and Laboratory Medicine

摘　　要：目的建立一种以病人新鲜全血为质控物的血细胞分析仪的质控方法。方法以Bayer AD-VIA2120血细胞分析仪为实验仪器,每天上午在配套质控物随标本测定后,选取20份结果合适的新鲜病人标本,下午重复测定1次(间隔6小时),连续检测10天,计算红细胞、白细胞、血红蛋白、红细胞压积、血小板前后2次检测结果的差值,将差值的均值和标准差定义为质控图的靶值和标准差,建立差值法Z分数质控图,再以Westgard质控规则连续17天监测病人新鲜全血标本,判断检测结果是否在控。结果除Hb发生1次失控外,差值法连续17天的质控结果均在控。结论差值法能作为仪器配套质控物8小时外质量控制的补充,该方法经济、方便,提高了检验报告的质量。Objective To set up a repetitive measurement difference value quality control method with patient fresh whole blood for hematocytes analysis. Method All tests were performed on Bayer ADVIA 2120 blood analyzer. 20 patient fresh blood samples were selected for 6 hours later detection after quality control passed which were checked among the patient samples every morning lasted for 10 days. The mean and standard deviation of repetitive measurement difference value of WBC, RBC, Hb, MCV and PLT were set as target value and standard deviation of Z-score charts, and then westgard rules were applied for the quality control for 17 days. Results Only Hb was happened 1 time out of control judged by difference value method, the others parameters were in control. Conclusion difference value method based on patient fresh whole blood is a financial and convenient method, which can be used as an appending method for routine matching control material quality assurance.

关 键 词：血细胞分析仪 质量控制 新鲜全血 Z分数图 

分 类 号：R446.111[医药卫生—诊断学] O213.1[医药卫生—临床医学]