深低温保存导致胎儿角膜细胞凋亡的初步研究  

Study of the effects of deep cryopreservation on apoptosis in fetal cornea

作　　者：马路生[1] 高波[1] 高磊[1] 毕宏广[1] 张春虹[1] 张秦娟[1] 王咏梅[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院眼科,中国山东省烟台市264000

出　　处：《国际眼科杂志》2008年第6期1137-1138,共2页International Eye Science

摘　　要：目的:研究新鲜和深低温保存后的胎儿角膜的细胞凋亡情况。方法:应用TUNEL法对20例新鲜及深低温保存后胎儿角膜进行了细胞凋亡的研究。结果:凋亡的角膜细胞全为细胞核着色。新鲜角膜中平均每高倍视野可见凋亡细胞0.817±0.612个,经冷冻保存后的角膜每高倍视野可见凋亡细胞平均为7.483±2.490个,经统计学处理P<0.05,差异有显著性。结论:新鲜胎儿角膜可见极少量凋亡细胞。胎儿角膜经深低温冷冻保存后,凋亡细胞数量显著增加。降温-复温损伤可能与细胞凋亡有关。AIM. To sum the number of apoptotic cells in both normal fetal cornea and the deep cryopreservative fetal cornea and to assess the effects of deep cryopreservation to apoptosis. METHODS： The TUNEL （TdT mediated biotin dUTP nick-end labeling, TUNEL） technique was used to study if apoptosis was appear in both nomal fetal cornea and cryopreservative cornea in 20 fetal cornea. RESULTS： The number of apoptotic cells was significantly higher after cryopreservation （7.483± 2. 490） than that of noncryopreservation （0.817±0.612,P〈0.05）. CONCLUSION ： Apoptosis is seldom seen in normal fetal cornea but is obvious in deep cryopreservative fetal cornea. That shows freeze-thaw trauma maybe relate to cell apoptosis.

关 键 词：凋亡 角膜 TUNEL 

分 类 号：R318.52[医药卫生—生物医学工程]