浙江省幽门螺杆菌cagA、vacA基因型的流行病学研究  

Molecular Epidemiologic Study of VacA Genotypes of Helicobacter Pylori in Zhejiang Province

作　　者：林朗[1] 杨恩[2] 张红河[2] 杨宁敏[2] 

机构地区：[1]浙江省苍南县人民医院,325800 [2]杭州致远医学检验所

出　　处：《医学研究杂志》2008年第6期94-96,共3页Journal of Medical Research

摘　　要：目的调查浙江地区幽门螺杆菌(helicobacter pylori,Hp)基因型的分布及其与临床疾病的关系。方法采用特异引物聚合酶链反应(PCR)分析262株幽门螺杆菌的vacA基因多态性分布特点,并对其进行初步统计分析。结果浙江8个地区分离培养的Hp菌株262株,VacA m1b基因型占27.10%(71/262);VacA m2基因型占64.89%(170/262);VacA m1b-m2基因型占4.20%(11/262);不同地区间VacA s区基因型和VacA m区基因型分布差异无统计学意义(P>0.05)。慢性胃炎分离株中VacA m1b基因型占26.47%(27/102);VacA m2基因型占66.67%(68/102);VacA m1bm2基因型占2.94%(3/102)。消化性溃疡分离株中VacA m1b基因型占29.41%(40/136);VacA m2基因型占61.76%(84/136);VacA m1bm2基因型占3.68%(5/136)。胃癌分离株中VacA m1b基因型占16.67%(4/24);VacA m2基因型占75.00%(18/24);VacA m1bm2基因型占4.17%(1/24)。不同临床疾病间VacA m区基因型分布差异无统计学意义(P>0.05)。结论浙江8个地区Hp菌株的优势基因型为cagA+、va-cAs1/m2,vacA的s区和m区的分布与分布个体的临床类型无关。Objective To investigate the the distribution of Helicobacter pylori （H. pylori） vacA gentypes and the relationship between the presence of specific genotypes and clinical diseases in Zhejiang of China. Methods 262 Helicobacter pylori strains were colleted from 8 districts of Zhejiang,chromosome DNA was extracted and polymerase chain reaction （PCR） was carried out to determine the polymorphism of vacA and cagA with specific primers. PCR results were analyzed statistically according to their isolated original and clinical outcomes. Results VacA mlb, vacAm2 and vacAml bm2 were found positive in 27.10% ,4.89% and 4.20% of the 262 H. Pylori strains respectively. There was no significant difference in vacA genotypes among different districts of Zhejiang. 26.47% （27/102） of vacA mlb,66.67% （68/102） vacAm2 and 2. 94% （3/102）vacAmlbm2 Helicobacter pylori strains were isolated from chronic gastritis; 29.41% （40/136） of vacA mlb, 61.76% （ 84/136 ） vacAm2 and 3.68% （ 5/136 ） vacAmlbm2 Helicobacter pylori strains were isolated from Peptic Ulcer;and 16.67% （4/24） of vacA mlb,75.00% （18/24） vacAm2 and 4.17% （1/24） vacAmlbm2 Helicobacter pylori strains were isolated from gastric cancer; There was no significant difference in vacA genotypes among different clinical disease. Conclusion CagA ＋ and vacAsl/m2 are predominant genotypes of H. Pylori in 8 districts of Zhejiang province. However, the relationship between vacA genotypes of H. pylori and the clinical disease can be identified in this study.

关 键 词：幽门螺杆菌 VACA基因型 流行病学 

分 类 号：R378.2[医药卫生—病原生物学] R446.5[医药卫生—基础医学]