自体骨髓间充质干细胞经皮注射修复骨缺损的实验研究  

An experimental study on percutaneous autologous bone marrow mesenchymal stem cell grafting for bone defect

作　　者：常玉立[1] 孙天胜[1] 张晓东[1] 马舟涌[1] 苏强[1] 任大江[1] 张立仁[1] 胥少汀[1] 

机构地区：[1]北京军区总医院骨科全军创伤骨科研究所,100700

出　　处：《中华医学杂志》2008年第23期1643-1647,共5页National Medical Journal of China

摘　　要：目的比较骨髓间充质干细胞（BMSCs）、成骨诱导后的BMSCs以及二者联合经皮移植后体内成骨能力的差异，为骨组织工程选择种子细胞提供依据。方法取第2代BMSCs进行成骨诱导。分别将BMSCs、成骨诱导的BMSCs、BMSCs联合成骨诱导后的BMSCs、生理盐水经皮注入兔桡骨中段骨缺损处。通过组织学、放射学、生物力学方法在不同时相比较骨缺损区修复情况。结果BMSCs与成骨诱导后的BMSCs联合移植组在12周内骨缺损区新生骨的数量和质量显著优于BMSCs移植组和成骨诱导的BMSCs移植组（F=226．6，P〈0．01），空白组骨缺损主要由纤维结缔组织填充。结论BMSCs与成骨诱导后的BMSCs联合移植存在协同效应，具有更强的成骨能力，可作为种子细胞应用于骨组织工程。Objectives To investigate in vivo osteogenic potential in size-critical bone defect after percutaneous autologous grafting of culture-expanded rabbit autologous BMSCs, osteo-induced BMSCs and combination of both. Methods BMSCs were cultured and then induced with osteogenic supplement （OS） medium. BMSCs with and without OS induction were collected and percutaneously autologously injected respectively into the 15 mm bone defect of 20 experimental rabbit model. The grafts were BMSCs, osteoinduced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, and 0. 9% NaCl solution. Osteogenesis at the defect areas were observed by regular radiography, histology and biomechanics. Results the group transplanted with BMSCs ＋ osteo-induced BMSCs achived complete bone healing with medullary cavity united, which showed the largest quantity of new bone measured by X-ray analysis, and also their maximal load were better than those in other groups. Conclusion The bone-forming ability of rabbit osteo-induced BMSCs combined with BMSCs in bone defect is superior to those of BMSCs and osteo-induced BMSCs.

关 键 词：骨髓移植 造血干细胞移植 骨缺损 

分 类 号：R686[医药卫生—骨科学]