脉冲磁场对多药耐药细胞K562/ADR的逆转作用  

Reversal effect of pulsed magnetic field on the multidrug-resistant cell line K562/ADR

作　　者：宋保民[1] 刘晓秋[1] 韩英[1] 赵阿津[1] 李德冠[1] 

机构地区：[1]中国医学科学院放射医学研究所,天津300192

出　　处：《医学研究生学报》2008年第6期568-571,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30570437)

摘　　要：目的:研究脉冲磁场(PMF)对多药耐药(MDR)肿瘤细胞K562/ADR的逆转作用及机制。方法:MTT法测定敏感株K562/S和耐药株K562/ADR对多柔比星(ADR)的IC50,观察PMF对肿瘤细胞增殖的影响。测定不同频率PMF照射的K562/ADR耐药细胞株对ADR的IC50,分析PMF的逆转效果。用流式细胞术(FCM)检测细胞内ADR的浓度。结果:K562/ADR细胞的耐药倍数为29.36,PMF对耐药株肿瘤细胞增殖无影响。相同磁场强度、不同频率的PMF对耐药株K562/ADR的IC50影响不同,在相同的时间里,70 Hz的PMF影响作用最明显。70 HzPMF处理的K562/ADR细胞中药物浓度高于对照组。结论:PMF可通过提高细胞内的药物浓度,对肿瘤细胞K562/ADR的MDR具有逆转作用;逆转作用的大小与脉冲磁场的频率有关,低频率的PMF比高频率的PMF的逆转效果更明显。Objective： To investigate the reversal effects of pulsed magnetic fields （PMF） on the drug resistant cell line K562/ADR in vitro and their mechanism. Methods： MTT assay was used to determine the IC50 of ADR on K562/S and K562/ ADR cell lines, the detriment of PMF on the K562/ADR cell line and the reversal effects of the drug resistance after treatment with different frequencies of PMF. Flow cytometry （FCM） was applied to the assessment of concentration of intracellular ADR. Results： The drug-resistance multiple of the K562/ADR cell line was 29.36. PMF did not affect the growth of the K562/ADR cell line. Different frequencies of PMF produced different effects on the IC50 of the K562/ADR cell line. In the same condition, 70 Hz PMF produced the most significant effect and in- creasea the concentration of intracellular ADR. Conclusion. PMF can reverse the multidrug resistance of K562/ADR by enhancing the drug concentration in K562/ADR cells, and its frequency is correlated with its reversal effect：low fregucncy produces better effect.

关 键 词：肿瘤 多药耐药 脉冲磁场 逆转 

分 类 号：R454.1[医药卫生—治疗学]