MAGE-3基因mRNA的表达在胸腔积液鉴别诊断中的价值  

The Value of MAGE-3 mRNA Expression in Pleural Effusion in Differential Diagnosis of Benign and Malignant Pleural Effusion

作　　者：刘静[1] 岳红梅[1] 胡泉[2] 

机构地区：[1]兰州大学第一医院呼吸科 [2]兰州大学第二医院神经外科,甘肃兰州730000

出　　处：《社区医学杂志》2008年第11期1-3,共3页Journal Of Community Medicine

摘　　要：目的研究MAGE-3基因mRNA在胸腔积液中的表达,探讨MAGE-3基因作为良、恶性胸腔积液鉴别诊断指标的价值。方法采用巢式逆转录-聚合酶链反应(nRT-PCR)方法,检测35例患者(25例恶性,10例良性)胸腔积液中MAGE-3的表达情况。结果10例良性胸腔积液组中,均未见有MAGE-3特异性条带;25例恶性胸腔积液组中,有14例可见MAGE-3特异性扩增条带,MAGE-3基因表达阳性率为56.00%,其敏感性、特异性、诊断符合率分别为56.00%、100.00%、68.57%。结论检测胸腔积液中MAGE-3基因mRNA的表达,可以作为鉴别良、恶性胸腔积液的一个指标。Objective This research was to detect whether the differential expression profile of the MAGE - 3 mRNA occurred in pleural effusion, and to explore the feasibility of MAGE - 3 gene detection as a diagnostic marker for benign and malignant pleural effusion. Methods MAGE -3 mRNA in pleural effusion was detected by nested RT- polymerase chain reaction （nRT-PCR） in 35 cases of patients （25 malignants and 10 benigns） complicating pleural effusion. Results No MAGE - 3 expression in pleural effusion of 10 patients complicating benign pleural effusion. As opposed to the benign controls, out of the 25 patients complicating ma- lignant pleural effusion, 14 ases of MAGE - 3 expression amplification strap were observed in pleural effusion and the rate of positive, sensitivity, specificity, accuracy were 56.00% ,56.00%, 100.00% ,68.57% to part. Conclusion To detect MAGE - 3 mRNA of pleural effusion might be a valuable diagnostic indicator for benign and malignant pleural effusion.

关 键 词：肺癌 MAGE-3基因 胸腔积液 巢式逆转录-聚合酶链反应 

分 类 号：R561.3[医药卫生—呼吸系统]