内质网应激联合线粒体在宫内窘迫胎鼠脑神经细胞凋亡中的作用研究  

Study of unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress

作　　者：卢敏[1] 张华[1] 漆洪波[1] 张建华[1] 

机构地区：[1]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2008年第6期704-707,共4页Journal of Chongqing Medical University

基　　金：重庆市自然科学基金(2004-5480);重庆市卫生局科研基金资助项目(05-2-189)

摘　　要：目的:探讨内质网和线粒体在胎鼠缺血缺氧再灌注损伤脑神经细胞凋亡中的联合作用。方法:制备宫内窘迫模型,将其随机分为正常组、缺血再灌注组。以Western blot技术检测葡萄糖调节蛋白78(Glucose regulated protein 78,GRP78),半胱氨酰天冬氨酸特异性蛋白酶(caspase)-9、caspase-12,细胞色素-c(CytochromeC)蛋白表达水平。结果:缺血再灌注组各时间点GRP78蛋白表达水平均高于正常组,于再灌注3h时达高峰,随再灌注时间延长,蛋白表达逐渐减低。缺血再灌注组各时间点caspase-9、caspase-12蛋白表达水平均高于正常组,均于再灌注12h达高峰。缺血再灌注组胞浆cytoronC蛋白表达水平在各个时间点均高于正常组,并于再灌注12h达峰值。结论:内质网和线粒体在宫内窘迫胎鼠脑神经细胞凋亡的发生过程中具有协同作用。Objective：To evaluae the unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress. Methods：Fetal rat intrauterine distress model was established. The samples were randomly divided into normal group and ischemia-reperfusion group（IR）. Expression of GRP78,caspase-9,caspase-12,and cytoron C protein were detected by western blot technique. Results：The expression of GRP78 on corresponding time in IR group was significantly higher than that in normal group. And it reached the maximum at 3h of reperfusion. The expression of GRP78 reduced step by step as reperfusion time extended. The expression of caspase-9 and caspase-12 at corresponding time in IR group were significantly higher than those in normal group. Their expression increased rapidly and reached the maximum at 12h of reperfusion. The expression of cytoron C in kytoplasm at corresponding time in IR group was significantly higher than that in normal group and reached the maximum at 12h of reperfusion. Conclusion：The synergistic effect of endoplasmic reticulum and mitochondrium plays an important role in fetal rat cerebral neuron apoptosis induced by intrauterine distress.

关 键 词：宫内窘迫 凋亡 内质网 线粒体 

分 类 号：R714[医药卫生—妇产科学]