兔血浆中顺式阿曲库铵的HPLC-荧光法测定  

Determination of cisatracurium in the plasma of rabbits by HPLC coupled with fluorescence detection

作　　者：周定利[1] 钟玲[1] 邱妍川[1] 雷光洪 尚京川[1] 

机构地区：[1]重庆医科大学药学院药物分析教研室,重庆400016 [2]重庆青阳药业有限公司,重庆400025

出　　处：《重庆医科大学学报》2008年第6期724-725,757,共3页Journal of Chongqing Medical University

摘　　要：目的:建立以反相高效液相色谱-荧光法测定兔血浆中顺式阿曲库铵(Cisatracurium)浓度的方法。方法:色谱柱为Agilent Hypersil ODS-C18柱(5μm,4.0mm×125mm),流动相为50mmol/L磷酸二氢钾缓冲液(pH3.0)-乙腈(50∶50),荧光检测的激发波长200nm、发射波长320nm。结果:顺式阿曲库铵检测浓度在25～2000ng/ml线性关系良好,r=0.9992。最低检测限为25ng/ml,日内精密度RSD≤2.56%,日间精密度RSD≤3.68%;提取回收率为85.2%～86.1%(n=5),加样回收率为93.4%～98.8%。结论:本方法简便,灵敏度高,重现性好,可满足药代动力学研究的需要。Objective：To determinate the concentration of Cisatracurium in the plasma of an rabbit via a proposed HPLC method. Methods：A C18 column was used,the mobile phase was made up of 50mmol/L dipotassium hydrogen phosphate（pH= 3.0） and acetonitrile（50：50）,and examination and emission wavelengths are 200nm and 320nm,respectively. Results：the linear range was 25-2 000ng/ml,the limit of detection（LOD） is 25ng/ml, the intra- and inter-precisions（RSD） were lower than2.56% and 3.68%,respectively. Besides,the recovery of extraction was 85.2%-86.1%（n=5） and the recovery of addition was 93.4% -98.8%. Conclusion：The proposed method proved to be accurate and repetitive.

关 键 词：顺式阿曲库铵 血药浓度 药代动力学 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]