用日本血吸虫重组抗原诊断牛血吸虫病的研究  

Studies on recombinant 23 ku membrane protein from Schistosoma japonicum as antigens in ELISA for serodiagnosis of bovine schistosomiasis

作　　者：周伟芳[1] 林矫矫[1] 朱传刚[1] 石耀军[1] 刘金明[1] 

机构地区：[1]中国农业科学院上海兽医研究所国家防治血吸虫病专业实验室农业部动物寄生虫学重点开放实验室,上海200232

出　　处：《中国兽医科学》2008年第6期489-493,共5页Chinese Veterinary Science

基　　金：国家"十五"科研院所社会公益研究专项(2004DIB4J158)

摘　　要：为寻找可替代虫卵抗原(SEA)用于家畜血吸虫病血清学诊断的重组抗原,采用酶联免疫吸附试验(ELISA)比较了日本血吸虫23ku膜蛋白大亲水区多肽重组蛋白LHD-Sj23和日本血吸虫SEA检测牛血吸虫病的敏感性和特异性。结果显示,SEA和LHD-Sj23作为诊断抗原对189例血吸虫病牛和92例健康牛的阳性检出率分别为87.8%和90.5%,阳性符合率分别为93.5%和92.4%。两种抗原之间敏感性和特异性均无显著性差异(P>0.05)。提示,LHD-Sj23重组抗原可替代SEA用于家畜血吸虫病的血清学诊断。To evaluate the potential of recombinant 23 ku membrane protein of Schistosoma japonicum （Sj23）-produced in the Escherichia coli as a substitute for soluble egg antigen（SEA） as coating antigen in serodiagnosis of bovine schistosomiasis, the sensitivity and specificity of recombinant Sj23 and SEA in ELISA were compared using 189 bovine sera with schistosomiasis and 92 bovine sera without schistosomiasis. The result showed that the sensitivity and specificity of ELISA using SEA as coating antigen were 87. 8% and 93.5%, respectively, and the sensitivity and specificity of ELISA using recombinant Sj23 as coating antigen were 90.5% and 92.4%,respectively. No significant difference was found between the two ELISAs on sensitivity and specificity,indicating that the recombinant Sj23 could be used as antigen to replace SEA in serodiagnosis of bovine schistosomiasis.

关 键 词：牛血吸虫病 重组抗原 血清学诊断 

分 类 号：S852.723[农业科学—基础兽医学]