741杨离体快繁和叶片再生体系的建立  

Rapid Micro-propagation and Establishment of Leaf Regeneration System of Poplar 741

作　　者：于学宁[1] 曹帮华[1] 曹玉翠[1] 王爱喜[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018

出　　处：《山东科学》2008年第1期35-38,共4页Shandong Science

基　　金：山东省农业良种工程项目计划资助(鲁科农字【2006】90号)

摘　　要：选用741杨的茎段和生根组培苗叶片为外植体,采用不同浓度组合的BA和NAA对741杨的离体快繁和叶片再生体系进行了研究。结果表明:741杨芽增值的最佳培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L;最佳生根培养基为MS+IBA 0.3 mg/L;741杨叶片不定芽诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L。试验还研究了不同着生位置的叶片以及叶片在培养基上的放置方式对741杨叶片再生的影响。Under the conditions of different concentrations of 6-BA and NAA, we employed such explants as stem segments and plantlet leaves of poplar 741 to investigate its micro-propagation and leaf regeneration system. Results indicate that the optimal medium for bud multiplication is MS ＋ 6-BA0.5 mg/L＋ NAA 0.1 mg/L; the best rooting medium is MS ＋ IBA 0.3 mg/L; the appropriate medium for shoot regeneration of a leaf is MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.1 mg/L. The experiment also studied the position effects of different leaves and the effects of placement of a leaf in media.

关 键 词：741杨 离体快繁 叶片再生 生根 

分 类 号：S722.37[农业科学—林木遗传育种]