应用PCR检测猪细小病毒  

Detection of Porcine parvovirus by PCR

作　　者：牛晖 艾伟昌[2] 陈付英[3] 邓大军[2] 

机构地区：[1]河南省肉牛工程技术研究中心,河南郑州450011 [2]河南省野生动物救护中心,河南郑州450044 [3]西北农林科技大学,陕西杨凌712100

出　　处：《郑州牧业工程高等专科学校学报》2008年第2期10-11,15,共3页Journal of Zhengzhou College of Animal Husbandry Engineering

摘　　要：根据GenBank已公布的猪细小病毒NADL-2株的基因序列,设计并合成了1对寡核苷酸引物,通过PCR扩增反应条件的优化.成功地从猪细小病毒感染的组织和细胞中扩增出882 bp片段。利用该方法对临床上的6份猪流产病料的检测,查出阳性4份。本试验建立的PCR诊断方法能快速、特异地诊断出猪细小病毒。A pair of primers was derived from the DNA sequences that have been published in GenBank. The PCR method was developed for the detection of PPV DNA. The results revealed that the expected 882 bp fragment could be amplified from many kinds of tissues of infected fetus and passaged cells, the Amplified fragment was show to be specific for PPV DNA. 4 positive of 12 samples from clinical sick swine were detected by PCR. In the present study,the method of PCR can rapidly ,specificly detect of PPV.

关 键 词：猪细小病毒 聚合酶链式反应 检测 

分 类 号：S852.659.6[农业科学—基础兽医学]