免疫印迹法分析不育症患者抗SPIM抗体  

ANALYSIS ON ANTI-SPIM ANTIBODIES IN INFERTILE PATIENTS BY WESTERN BLOTTING

作　　者：黄邱朝[1] 商学军[1] 黄宇烽[1] 

机构地区：[1]南京军区总医院全军医学检验中心,210002

出　　处：《皖南医学院学报》1997年第4期326-328,共3页Journal of Wannan Medical College

摘　　要：采用聚丙烯酰胺凝胶电脉和免疫印迹法分析不育症患者抗精浆免疫抑制物抗体（SPIM—Ab）对特异抗原的免疫反应，并与ELISA法进行比较。结果表明，免疫印迹法检测血清、精浆SPIM—Ab阳性率分别为30．0％（32／107）和29．0％（36／124），ELISA法检测分别为33．6％（36／107）和31.5％（39／124），两法差异无显著性（P＞0．05）。免疫印迹法分析的68份不育症患者的血清、精浆SPIM—Ab阳性标本中，49份（72．1％）与特异抗原的110KD和（或）94KD区带发生免疫反应，19份（27．9％）与52KD区带反应，13份（19．1％）与36KD区带反应。提示，大分子量SPIM抗原可能更容易诱导产生高效价SPIM—Ab以干扰生育。Immunologic reaction to specific antigens of antiseminal plasma immunosuppresive material antibodies(SPIM-Ab) in infertile patients was analyzed by SDS-PAGE and Western blotting , and compared with ELISA. The results showed that determinated by Western blotting and ELISA,the positive rates of SPIM-Ab in serum and seminal plasma were 30. 0% (32/ 107 ), 29. 0% (36/124)and 33. 6 % (36/107), 31. 5 % (39/ 124 ). respectively, there were no satistical significance (P >0. 05). 49(72. 1 % )samples were reacted to 110 KD and 94KD bounds of SPIM specific antigens,19(27. 9% ) and 13(19. 1 % ) samples were reacted to 52 KD and 30KD bounds of this antigens, respectively, in 68 samples with positive SPIM-Ab in serum and seminal plasma of infertile patients. It suggests that the high titer SPIM-Ab may be easily induced by the high molecular weight spim antigens and interfere fertility.

关 键 词：免疫印迹 不育症 SPIM 抗体 ELISA 

分 类 号：R698.204[医药卫生—泌尿科学] R446.61[医药卫生—外科学]