延胡索及其破壁品、炮制品中有效成分的研究  

Studies on active ingredients in corydalis,Broken cell wall corydalis and its processed products

作　　者：曹柳[1] 窦志英[1] 王萍[1] 孙巍[1] 田永亮[1] 

机构地区：[1]天津中医药大学中药炮制教研室,天津300193

出　　处：《中国中药杂志》2008年第11期1263-1265,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672664);天津市高等学校科技发展基金项目(20040309)

摘　　要：目的:比较生品、破壁延胡索及其炮制品中延胡索乙素和去氢紫堇碱的含量差异。方法:采用破壁技术对延胡索进行破壁处理并炮制,采用Kromasil C18色谱柱(4.6 mm×250 mm,5.0μm),流动相乙腈-醋酸盐缓冲液(pH6.0)(30∶70),流速1 mL.min-1,柱温35℃,检测波长280 nm,测定破壁及炮制前后的延胡索乙素和去氢紫堇碱的含量。结果:破壁延胡索与生品相比,去氢紫堇碱和乙素含量都有所增高,破壁醋炙品中两者含量最高。结论:延胡索破壁后有利于生物碱的溶出。Objective： To compare the contents of tetrahydropalmatine and dehydrocorydaline in corydalis, broken cell wall co- rydalis and its different processed products. Method： The broken cell wall technique was used to corydalis, and then both the corydalis and broken cell wall corydalis were processed. The method of chromatography which was used to determine the contents of tetrahydropalmatine and dehydrocorydaline in corydalis broken cell wall corydalis and its different processed products was performed by RP-HPLC with Kromasil ODS-CIs （4. 6 mm ×250mm,5.0μm） column was used at 35 ℃, acetonitrile-acetate buffer solution （pH 6.0 ） （30：70 ） as mobile phase of 1 mL· min - 1 flow rate, detection wavelength was set at 280 nm. Result： The contents of the two active components in broken cell wall corydalis were higher than that in corydalis, while that in broken cell wall and vinegar-fried corydalis was highest in the products of brearked cell wall corydalis. Conclusion： Breaking the cell wall of corydalis can help to dissolute alkaloids.

关 键 词：破壁延胡索 延胡索乙素 去氢紫堇碱 HPLC 

分 类 号：R284[医药卫生—中药学]