远志抗抑郁有效部位中寡糖酯单体的分离及活性研究  

Study on antidepressant components of sucrose ester from Polygala tenuifolia

作　　者：涂海华[1] 刘屏[1] 马亮[1] 廖红波[1] 谢婷婷[1] 穆丽华[1] 刘耀明[2] 

机构地区：[1]解放军总医院临床药理研究室,北京100853 [2]江西中医学院,江西南昌330006

出　　处：《中国中药杂志》2008年第11期1278-1280,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572354)

摘　　要：目的:研究远志的抗抑郁活性成分。方法:用色谱法分离远志的化学成分,根据波谱学分析鉴定了化合物的结构,用MTT法评价其细胞毒活性。结果从远志分离得到8个化合物,分别鉴定为:西伯利亚远志糖A1(sibiricose A1,1),西伯利亚远志糖A5(sibiricose A5,2),tenuifoliside A(3),西伯利亚远志糖A6(sibiricose A6,4),3,4,5-三甲氧基肉桂酸甲酯(3,4,5-trimethoxycinnamate,5),远志酮Ⅲ(polygalaxanthoneⅢ,6),tenuifolioses A(7),tenuifolioses H(8)。结论:化合物1为首次从远志中分离得到,远志中的寡糖酯类成分对谷氨酸造成的PC12细胞损伤均有一定的保护作用。Objective： To investigate the antidepressant components of Polygala tenuifolia. Method： The chromatographic method was used to isolate and purify the chemical constituents, their structures were identified by spectral analysis, MTT method was applied to investigate their cytotoxic activities. Result： Nine compounds were isolated from the roots of P. tenuifolia. Their structures were identified as sibiricose A, （1）, sibiricose A5 （2）, tenuifoliside A （3） and 3＇, 6-disinapoyl sucrose （4）, sibiricose A6 （5）, 3, 4, 5-trimethoxycinnamate （6）, polygalaxanthone Ⅲ （7）, tenuifolioses A （8）, tenuifolioses H （9） and some compounds＇activities to PC12 were observed. Conclusion： Compound 1 was isolated from this plant for the first time. Compounds 2,3 could protect PC12 cells damage induced by P. tenuifolia.

关 键 词：远志 寡糖酯 PCI2细胞 抗抑郁 

分 类 号：R284.1[医药卫生—中药学]