超滤法测定甲基原薯蓣皂苷的血浆蛋白结合率  

Determination of plasma protein binding rate of methyl protodioscin with ultrafiltration

作　　者：姚志红[1] 曹秀珍[2] 邵萌[3] 潘宇明[1] 叶文才[1] 姚新生[1] 

机构地区：[1]暨南大学中药及天然药物研究所,广东广州510632 [2]沈阳药科大学天然药物化学教研室,辽宁沈阳110016 [3]中国药科大学中药学院,江苏南京210009

出　　处：《中国中药杂志》2008年第11期1291-1294,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30300432)

摘　　要：目的:测定甲基原薯蓣皂苷的血浆蛋白结合率。方法:采用超滤法结合高效液相色谱-串联质谱联用法(HPLC-MS-MS)对甲基原薯蓣皂苷与大鼠血浆以及健康人血浆的蛋白结合率进行测定。结果:甲基原薯蓣皂苷与大鼠血浆在20.0,100,200μg.mL-1下的血浆蛋白结合率分别为(94.6±0.16)%,(91.6±0.35)%,(86.10±0.60)%。甲基原薯蓣皂苷在以上3个浓度下与健康人血浆的血浆蛋白结合率分别为(82.11±5.12)%,(84.54±0.32)%,(88.52±1.02)%。结论:甲基原薯蓣皂苷与血浆蛋白具有较强的结合。Objective：To study the plasma protein binding rate of methyl protodioscin. Method： The ultrafiltration was employed to determine the plasma protein binding rate of methyl protodioscin. The plasma concentrations of methyl protodioscin were measured by HPLC-MS-MS. Result：The plasma protein binding rate of methyl protodioscin with rat plasma at the concentration of 20. 0, 100 and 200 μg·mL-1 were （94. 6 ± 0. 16 ） % , （91.6 ± 0. 35 ） % and （86. 10 ± 0. 60 ） %, respectively, while the plasma protein binding rate of methyl protodioscin with normal human plasma at the above concentrations were （82. 11 ± 5.12 ）%, （84. 54 ± 0. 32）% and （88.52 ± 1.02）%, respectively. Conclusion： The binding rate of methyl protodioscin with plasma protein is high.

关 键 词：甲基原薯蓣皂苷 血浆蛋白结合率 超滤法 HPLC—MS-MS 

分 类 号：R285.5[医药卫生—中药学]