艾灸治疗对兔膝关节骨性关节炎调节作用的实验研究  

Regulative effect of moxibustion on experimental knee osteoarthritis in rabbit

作　　者：郑曙光[1] 陈波[1] 马四补[1] 李佳霖[1] 谢西梅[1] 

机构地区：[1]贵阳中医学院,贵州550002

出　　处：《北京中医药大学学报》2008年第5期358-360,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：贵州省科技攻关项目(No.黔科合S字2007[1054])

摘　　要：目的探讨艾灸治疗对膝关节骨性关节炎(KOA)软骨蛋白多糖和Ⅱ型胶原表达的调节作用,为艾灸临床治疗KOA提供实验依据。方法通过兔膝关节注射木瓜蛋白酶制造KOA模型,经艾灸和药物治疗6周后,采用定量图像分析技术检测膝关节软骨中蛋白多糖、Ⅱ型胶原、白细胞介素1β(IL-1β)和基质金属蛋白酶(MMP-1)含量的改变。结果艾灸治疗能够增加关节软骨蛋白多糖和Ⅱ型胶原含量,降低IL-1β和MMP-1的表达,疗效与药物组相似。结论艾灸能够起到减轻KOA软骨破坏、减轻关节病损的作用,其作用机制可能是通过抑制关节炎症,减少炎症介质IL-1β对MMP-1的激活作用实现的。Objective To explore the regulative effect of moxibustion on the expression of proteoglycan and collagen Ⅱ in cartilage of rabbit with experimental knee osteoarthritis（KOA） to providing experimental basis for curing KOA with moxibustion.Methods The KOA animal model was built up by injecting papain into knee joint of rabbit.After 6 weeks of treatment with moxibustion and medication respectively,changes of the contents of proteoglycans,collagen Ⅱ,IL-1β and MMP-1 were detected and analized by quantitative image analyzer technique.Results The contents of proteoglycans and collagen Ⅱ were increased by treatment of moxibustion,but the expression of IL-1β and MMP-1 were restrained.Similar changes happened in the group of medication.There were no statistical significances between the moxibustion treatment and the medication treatment.Conclusion Moxibustion could lighten the impairment of cartilage of KOA,and then the serious degree of KOA was alleviated.The possible mechanism of action was that moxibustion could restrain the inflammation in joints and reduce the activation of MMP-1 induced IL-1β.

关 键 词：膝关节骨性关节炎 艾灸 蛋白多糖 Ⅱ型胶原 白细胞介素1Β 基质金属蛋白酶1 兔 

分 类 号：R245.81[医药卫生—针灸推拿学]