外源性人细胞因子对离体培养的晚孕绒毛组织产生βhCG的影响  

CYTOKINE MODULATION OF βHCG PRODUCTION IN CULTURED PLACENTA VILLI FROM LATE PREGNANCY

作　　者：麻莉[1] 丁晓萍[1] 刘蕊[1] 袁戈[1] 孙建华[2] 

机构地区：[1]第二炮兵总医院妇产科,北京100088 [2]第二炮兵总医院检验科,北京100088

出　　处：《北京师范大学学报（自然科学版）》2008年第3期299-301,共3页Journal of Beijing Normal University(Natural Science)

摘　　要：利用体外培养的晚期胎盘绒毛组织,加入重组人白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNFα),放射免疫法测定培养绒毛组织上清液中βhCG质量浓度,免疫组化法检测培养绒毛组织中的βhCG表达.观察外源性细胞因子IL-1I、L-6、TNFα对离体培养的晚孕期绒毛组织产生βhCG的影响,探讨细胞因子与βhCG水平改变的关系.结果表明在离体培养的晚期妊娠绒毛中加入重组细胞因子后,滋养细胞产生βhCG的量增加,尤其在加入较高浓度的rhIL-6、rhTNFα时βhCG的产生量增加更明显.免疫组化染色结果显示:rhIL-1、rhIL-6、rhTNFα处理组滋养细胞βhCG表达的阳性程度均高于对照组.说明在炎性细胞因子的作用下,晚期妊娠滋养细胞产生的βhCG增加.To study cytokine modulation of βhCG production in cultured placenta villi from late pregnancy. βhCG content was determined by radioimmunoassay and in cultured villus by immunohisto-chemistry. When recombinant cytokines were added, βhCG production was increased markedly, especially so with high doses of human IL-6 and TNFα. These data suggest that inflammatory cytokines stimulate βhCG production in late pregnancy placenta.

关 键 词：Β人绒毛膜促性腺激素 外源性细胞因子 绒毛 

分 类 号：Q813.11[生物学—生物工程] TQ464[化学工程—制药化工]