间隔区寡核苷酸分型法用于99株结核分枝杆菌杭州临床分离株的基因分型研究  

Genotyping of 99 clinical isolates of Mycobacterium tuberculosis using spoligotyping in Hangzhou

作　　者：王勐[1,2] 刘志广[1] 赵秀琴[1] 董海燕[1] 刘洁[1] 张媛媛[1] 吕冰[1] 万康林[1] 

机构地区：[1]传染病预防控制国家重点实验室,中国疾病预防控制中心传染病预防控制所,北京昌平102206 [2]杭州市疾病预防控制中心

出　　处：《疾病监测》2008年第5期300-303,323,共5页Disease Surveillance

基　　金：国家自然科学基金资助项目(30471526)

摘　　要：目的用间隔区寡核苷酸分型(Spoligotyping)鉴定杭州结核分枝杆菌临床分离株的基因分型种类和特征,为结核病防治研究提供基础科学依据。方法收集结核分枝杆菌杭州临床分离株,应用Spoligotyping进行基因分型检测。基因聚类分析采用BioNumerics(Version 5.0)软件。结果共在杭州市收集到99株结核分枝杆菌临床分离株,可分为2个基因群,即北京家族(Beijing family)和非北京家族(Non-Beijing family),分别占64.65%(64/99)和35.35%(35/99),20种基因型,其中9株为独特类型,剩余90株分为11簇。北京家族菌株中,89.06%(57/64)为典型北京家族(Typical Beijingfamily),35株非北京家族菌株可分为16个基因型,10株为独特的基因型。结论杭州结核分枝杆菌存在明显的基因多态性,北京家族菌株占优势,但非北京家族菌株基因多态性更显著。Objective The present study was conducted to identify the genotypes and species of Mycobacterium tuberculosis （M. tuberculosis） strains isolated in Hangzhou, the spacer oligonucleotide typing （Spoligotyping） was employed in the present study, providing evidence for the prevention and management of tuberculosis （TB）. Methods M. tuberculosis clinical strains isolated from patients with tuberculosis （TB） in Hangzhou were collected and genotyped with spoligotyping. Gene cluster analysis was performed using BioNumerics （Version 5.0） software. Results A total of 99 clinical isolates of M. tuberculosis were collected in Hangzhou, which were divided into the Beijing family and the non- Beijing family by means of Spoligotyping, accounting for 64.65% （64/99） and 35.35% （35/99）, respectively. A total of 20 genotypes were found, including 9 unique ones and 11 clusters that contained the other 90 strains. As to the Beijing family, 89.06%（57/64） of the strains belonged to the typical Beijing family; the 35 strains from the non-Beijing family could be subdivided into 16 genotypes with 10 unique ones. Conclusion Strong allelic diversity of M. tuberculosis in Hangzhou was found. The Beijing family predominated, though polymorphism in the non-Beijing family was more remarkable.

关 键 词：结核分枝杆菌 间隔区寡核苷酸分型 基因分型 北京基因型 

分 类 号：R450[医药卫生—治疗学]