枳术汤加味对大鼠肠黏膜屏障功能的保护作用  

The mechanism of protective effect of the Zhizhu decoction on rat intestinal mucosal barrier

作　　者：张家衡[1] 刘彦[1] 陈奇[1] 金太欣[1] 辛玲芳[1] 

机构地区：[1]武汉市中西医结合医院武汉市第一医院外科,430022

出　　处：《中华实验外科杂志》2008年第6期758-760,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨中药枳术汤加味对大鼠肠黏膜屏障功能的保护作用及其机制。方法将70只雄性大白鼠随机分为对照组（10只）、造模组（20只）术前连续7d饮水灌胃0．35ml／次，每天2次，治疗组（20只）术前连续7d中药灌胃0．35ml／次，每天2次，大剂量治疗组（20只）术前连续7d中药灌胃0．7ml／次，每天2次。大鼠按2．5mL／kg体重的剂量给予腹腔内注射5％水合氯醛进行麻醉。进腹后血管钳夹闭肠系膜上动脉，持续30min后恢复血供，使肠道发生缺血-再灌注损伤。分别在再灌注1、24h后进行以下检测：肠道细菌移位发生率；回肠黏膜病理变化；外周血浆D-乳酸浓度、二胺氧化酶浓度；回肠黏膜细胞凋亡指数。结果缺血-再灌注1、24h后细菌移位阳性率、细菌培养阳性率和血浆D-乳酸和二胺氧化酶浓度，造模组高于对照组（P〈0．05）；治疗组低于造模组（P〈0．05）；大剂量治疗组和治疗组比较差异无统计学意义（p〉0．05）。回肠膜黏重量、绒毛高度及宽度，造模组小于对照组（P〈0．05）；治疗组大于造模组（P〈0．05）；大剂量治疗组和治疗组比较差异无统计学意义。肠黏膜上皮细胞凋亡指数，造模组高于对照组（P〈0．05）；治疗组低于造模组（P〈0．05）；大剂量治疗组和治疗组比较差异无统计学意义（P〉0．05）。结论中药枳术汤加味能减少肠道细菌移位，降低肠黏膜的通透性，可以保护肠黏膜屏障功能。Objective To study the mechanism of protective effect of the Chinese traditional medicine Zhizhu decoction on rat intestinal mucosal barrier. Methods According to random number table,70 male rats were divided into control group （10） ,model group （20） ,treatment group （20） and the high-dose treatment group （20）. The latter three groups were given water or decoction 2 times a day by intragastric administration for 7 days before operation ： the model group was administered with water 0.35 ml one time ; the treatment group with decoction 0.35 ml one time and the high-dose treatment group with decoction 0.7 ml one time. Rats were subjected to intraperitoneal injection of 5% chloral hydrate （2.5 ml/ kg） for anesthesia. Vascular clamp was used to close the upper mesenteric artery after opening the abdominal cavity, then blood supply was restarted after continuing 30 min and intestinal ischemia and reperfusion injury was made. Following measures 1 and 24 h after reperfusion were done,respectively： （ 1 ） Incidence of intestinal bacterial translocation; （2） Ileal mucosa pathological changes; （3） Peripheral blood plasma D-lactate concentration, diamine oxidase concentration; （4） Heal mucosa apoptosis index. Results After ischemia - repel＇fusion for 1 h or 24 h, the bacterial transloeation positive rate, bacterial culture positive rate, and plasma D-lactate and diamine oxidase concentration of model group were higher than in control group （ P 〈 0.05 ） ; those indexes of the treatment group were lower than in model group （ P 〈 0.05 ）. Those indexes had no significant difference between the high-dose treatment group and the treatment group. The weight of ileal mucosa and the height and width of villus in the model group were decreased as compared with control group （ P 〈 0.05 ） ; those indexes in the treatment group were increased as com- pared with model group （ P 〈 0.05 ）. Those indexes had no significant difference between the high-dose treatment group and th

关 键 词：肠黏膜 屏障 枳术汤加味 

分 类 号：R285.5[医药卫生—中药学]