RGD肽对猪去细胞心脏瓣膜表面修饰的研究  

Experiment on surface modification of porcine decellularized valve scaffold with RGD peptide

作　　者：史嘉玮[1] 董念国[1] 孙宗全[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管外科,武汉430022

出　　处：《中华实验外科杂志》2008年第6期777-778,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30571839、30600608）

摘　　要：目的检测促黏附分子RGD肽（精-甘-天冬氨酸）表面修饰去细胞瓣天然支架可行性。方法应用酶和去污剂法制备猪去细胞主动脉瓣，固相法合成含RGD短肽GRGDSPC。实验组采用化学交联剂Sulfo-LC—SPDP使GRGDSPC肽与之结合，去细胞瓣直接混合短肽和单纯去细胞瓣两组作对照。X射线光电子能谱法（XPS）支架材料表面分析。结果XPS法显示，对照组未检出硫元素，实验组检出硫元素电子结合能中间峰位在163．1～165．7eV，提示二硫键形成，证明Sulfo-LC—SPDP使GRGDSPC肽成功固定于去细胞瓣表面。结论通过化学交联法实现RGD肽对去细胞瓣表面修饰，有望促进组织工程心脏瓣膜构建。Objective To modify decellularized valve scaffold with arginine -glycine -aspartic acid （RGD） containing peptides to promote cell attachment. Methods Porcine aortic valves were decellularized with a protocol of trypsinase ＋ detergent Triton-100. GRGDSPC peptide was synthesized with the method of solid phase. With the help of a cross-linker Sulfo-LC-SPDP, decellularized valve was immobi- lized with GRGDSPC peptide in the coupled group. The simple coated and untreated decelluarized valves were classified into controls. Then X-ray photoelectron spectroscopy （XPS） was used for structure analysis. Results According to XPS spectra in the coupled greup,the sulfur element and disulfide bonds were found in the range of 163.1-165.7 eV. Therefore RGD peptides and decelluarized valves could be covalently bound by this way. Conclusion It is feasible and beneficial for immobilization of decellularized valve scaffold with RGD peptides to construct tissue engineering heart valves.

关 键 词：RGD肽 瓣肽 生物支架 表面修饰 

分 类 号：R318.08[医药卫生—生物医学工程]