Interaction Between Cytochrome c and the Hapten 2,4-Dinitro- fluorobenzene by Electrospray Ionization Mass Spectrometry  

作　　者：Bo Wu Yan-qiu Chu Zhao-yun Dai Chuan-fan Ding 

机构地区：[1]Physical Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China [2]Infectious Disease Department, Huadong Hospital, Shanghai 200040, China

出　　处：《Chinese Journal of Chemical Physics》2008年第3期217-220,共4页化学物理学报（英文）

基　　金：This work was supported by the National Natural Science Foundation of China （No.20473020）.

摘　　要：Allergic contact dermatitis is a delayed hypersensitivity reaction, which results from skin exposure to low molecular weight chemicals such as haptens. To clarify the pathogenic mechanism, electrospray ionization mass spectrometry （ESI-MS） and hydrogen/deuterium （H/D） exchange, as well as UV spectroscopy, were applied to determine the interaction between the model protein cytochrome c （cyt c） and the hapten 2,4- dinitro-fiuorobenzene （DNFB）. The ESI-MS results demonstrate that the conformation of cyt c can change from native folded state into partially unfolded state with the increase of DNFB. The equilibrium state H/D exchange followed by ESI-MS further confirms the above results. UV spectroscopy indicates that the strong- field coordination between iron of heme （prosthetic group） and His18 or Met80 of cyt c is not obviously affected by the hapten.

关 键 词：INTERACTION Cytochrome c 2 4-dinitro-fluorobenzene ESI-MS 

分 类 号：O64[理学—物理化学]