碳纳米管／TiO2薄膜固定酶的生物传感器及电催化氧化H2O2的研究  

Electrochemical oxidation of hydrogen peroxide at a HRP biosensor modified with TiO_2-decorated carbon nanotubes

作　　者：时巧翠[1] 王素芬[2] 

机构地区：[1]浙江警察学院,杭州310053 [2]浙江大学化学系,杭州310028

出　　处：《分析试验室》2008年第6期5-8,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然壳学基金(29975024,20275034)项目资助

摘　　要：采用酸性溶胶法在碳纳米管上负载纳米TiO2颗粒,并制备了CNT-TiO2薄膜固定辣根过氧化物酶的生物传感器。研究了该生物传感器在磷酸盐缓冲溶液中对H2O2的电催化氧化作用及实验条件的影响。结果表明该生物传感器对H2O2表现出良好的电催化性能,在最佳实验条件下,对H2O2检测的线性范围为:4.2×10-7mol/L^3.2×10-3mol/L。检出限为:7.22×10-8mol/L。The acid sol method was used for decorating TiO2 nanoparticles onto carbon nanotube surface. A gold elec- trode modified with TiO2-CNT was prepared. An amperometric horseradish peroxidase biosensor was prepared by immobilization of HRP in TiO2-CNT thin film on the gold electrode. The electrochemical oxidation of hydrogen peroxide was studied on this biosensor. The experiments showed that the nanoparticles could enhance the catalytic activity of immobilized enzyme. The linear detection range was 4.2 × 10^-7 mol/L - 3.2 × 10^-3 mol/L and the detection limit was 7.22 × 10^-8 mol/L.

关 键 词：生物传感器 CNT-TiO2 辣根过氧化物酶 化学修饰电极 

分 类 号：O657.1[理学—分析化学]