流动注射电化学发光法测定盐酸麻黄碱  

Determination of ephedrine hydrochloride in pharmaceutical samples by a flow-injection electrochemiluminescence method

作　　者：柴晓莉[1] 赵常志[1] 陶晟辰[1] 李明华[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《分析试验室》2008年第6期108-111,共4页Chinese Journal of Analysis Laboratory

摘　　要：基于盐酸麻黄碱(EP)对三联吡啶合钌(Ru(bpy)32+)电化学发光(ECL)的增敏作用,建立了流动注射ECL检测EP的新方法,并将其应用于EP的测定。结果表明,在pH为10.0的0.1 mol/L Na2B4O7-NaOH介质中,在电位1.10 V下进行恒电位电解,Ru(bpy)32+的浓度为1.0×10-4mol/L时,EP对ECL的增敏效果最好。在优化的条件下,测定EP的线性范围为2.40~24.0μg/mL(r=0.9995),检出限为2.00μg/mL,相对标准偏差(RSD)小于1.6%(n=10),加标回收率为97.0%~105%。A new flow-injection el （ECL） analytical method of ephedrine hydrochloride has been proposed in this paper. It is based on a fact that ephedrine could react with tris （2,2′-bipyridine）ruthenium（ Ⅱ ） （Ru（bpy）3^2＋ ） to enhance electrochemiluminescence signal. In the Na2B4O7-NaOH solution of pH 10.0, the best ECL response was obtained at a potential of 1.10 V in the present of 1.0 × 10^-4 mol/L Ru（bpy）3^2＋ . The linear range of the method is from 2.40 to 24.0 μg/mL with a correlation coefficient of 0.9995 and a detection limit of 2.00 μg/mL. The method has successfully applied to the determination of ephedrine hydrochloride in ephedrine injection and nasal drop. The relative standard deviation （R.S.D.） was less than 1.6% （n = 10） and recoveries ware from 96% to 105% for the dete^nination of practical samples.

关 键 词：盐酸麻黄碱 电化学发光 流动注射分析 三联吡啶合钌 

分 类 号：O657.3[理学—分析化学]