甲苯胺蓝循环伏安法测定硫酸软骨素  

Cyclic Voltammetry Determination of Chondroitin Sulfate with Toluidine Blue

作　　者：勾华[1] 罗红群[1] 李念兵[1] 

机构地区：[1]西南大学化学化工学院

出　　处：《西南大学学报（自然科学版）》2008年第5期58-61,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20575054);重庆市科委资助项目(CSTC-2006BB0342)

摘　　要：在pH=4．8的Britton-Robinson（B-R）缓冲溶液中，用循环伏安法研究了甲苯胺蓝（TB）与硫酸软骨素（cs）的相互作用．在最佳实验条件下，TB与CS通过静电作用形成复合物，导致溶液的氧化峰电流和还原峰电流均明显降低，其峰电流差值（△ip）与CS的浓度成正比，据此建立了一种测定CS浓度的新方法，测定的线性范围为1．0～21．0μg／mL，相关系数R=0．9981，检测限（3σ）为0．26μg／mL．该法应用于硫酸软骨素针剂的测定，结果满意．The interaction between toluidine blue （TB） and chondroitin sulfate （CS） was studied by cyclic voltammetry in Britton-Robinson buffer solution at pH 4. 8. Under the optimum conditions, TB reacted with CS to form a complex compound by virtue of electrostatic force, which resulted in significant decrea- ses in both the oxidation peak current and the reduction peak current. The difference value of peak currents （△ip） was proportional to the concentration of CS. Based on that, a new method for the determination of CS concentration was established, with a linear range of 1.0--21.0 mg/mL for CS determination, a correlation coefficient of 0. 998 1 and a determination limit of 0. 26 mg/mL. The method was applied to CS determination of CS injection samples with satisfactory results.

关 键 词：硫酸软骨素 甲苯胺蓝 循环伏安法 

分 类 号：O657.1[理学—分析化学]