培养基渗透压对延缓生长的苹果组培苗生理代谢指标影响  

Effect of Osmotic Pressure in Media on Physiological Indices of Apple Explants in vitro for Retardant Growth Conservation

作　　者：王晨[1] 师校欣[1] 杜国强[1] 黄瑞虹[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001

出　　处：《中国农学通报》2008年第6期222-225,共4页Chinese Agricultural Science Bulletin

基　　金：河北省自然科学基金资助项目"不同继代次数苹果组培苗种质保存特性研究"(C2008000287)

摘　　要：以富士、乔纳金苹果试管苗为试材,研究了不同渗透压处理下苹果组培苗延缓生长以及恢复生长后SOD、POD活性和MDA含量的变化,以期为筛选苹果组培苗延缓生长种质保存的适宜方法提供依据。结果表明:在延缓生长保存期间,随培养基中蔗糖、琼脂浓度提高,添加甘露醇,试管苗SOD、POD活性逐渐升高,而MDA含量呈下降趋势,依品种不同分别在蔗糖浓度达到50～60g/L、琼脂浓度达到10～14g/L、甘露醇浓度达到30～40g/L时达显著差异水平。恢复生长当代试管苗的SOD活性、POD活性以及MDA含量处理间表现相同。研究认为添加50～60g/L的蔗糖适宜苹果试管苗延缓生长法种质保存。It was studied that the changes of SOD and POD activity and MDA amount in Fuji and Jonagold apple explants in vitro during conservation period in subculture media under certain osmotic pressures and recovery process in order to optimize the culture condition for retardant growth. The results showed that the SOD and POD activity increased and MDA amount decreased gradually along with the enhancing of the concentration of Sucrose,Agar,and Mannitol in subculture media during conservation period. The significant difference was found when the concentration was reached 50-60g/L of Sucrose,10-14g/L of Agar,and 30-40g/L of Mannitol,depending on varieties. This difference disappeared when the explants were transferred to the conventional medium for recovery growth culture. It suggests that the supplying of 50-60 g/L Sucrose in a medium is suitable for retardant growth conservation in apple.

关 键 词：渗透压 苹果组培苗 POD活性 SOD活性 MDA含量 

分 类 号：S661[农业科学—果树学]