磁性纳米粒子的制备及其细胞分离方面的应用  

Preparation of magnetic nano-particles and their application in cell separation

作　　者：张春明[1] 赵梗明[2] 斯庆苏都[1] 谢湘华[3] 谭玲[1] 董亚明[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海师范大学数理信息学院,上海200234 [3]华东理工大学理学院,上海200237

出　　处：《上海师范大学学报（自然科学版）》2008年第3期291-295,共5页Journal of Shanghai Normal University(Natural Sciences)

摘　　要：介绍了一种始终在溶液中制备Fe3O4磁性纳米粒子的化学共沉淀,并对制得的粒子进行表面修饰的方法.通过IR,XRD和AFM等测试仪器对样品进行了表征.结果表明:采用化学共沉淀的方法制备出的磁性纳米粒子具有单分散性,且粒径比较均一,约在30nm.由于制备过程均在液体中进行,故制备周期短,污染小.同时利用自制的免疫磁性Fe3O4纳米粒子能较好地分离脐血中的CD133细胞,细胞体外扩增明显.Ferriferous oxide nano-particles were prepared using chemical co-precipitation method and then the particles were modified by chemical reagent and the whole process was carried out in a solution. Infrared spectra, X - ray diffraction and atomic force microscope were applied to characterize the magnetic nano-particles. The results indicate that the well dispersed, non-polluted and bio-compatible nano-particles were obtained with their average diameter of around 30 nm. CD133 cells were well separated using this kind of magnetic nano-particles and a good increment was obtained.

关 键 词：纳米FE3O4 免疫磁性纳米粒子 脐带血 CD133细胞 

分 类 号：TQ138.1[化学工程—无机化工]