卡式微柱凝胶法在猴血型鉴定中的应用  

Evaluation of the Gel System for ABO Typing of Monkeys

作　　者：陈松[1] 魏晴[2] 李俊华[1] 向莹[1] 郭晖[1] 王婉瑜[3] 熊郁良[3] 陈实[1] 陈刚[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所,湖北武汉430032 [2]华中科技大学同济医学院附属同济医院血库,湖北武汉430032 [3]中国科学院昆明动物研究所动物毒素室,云南昆明650223

出　　处：《Zoological Research》2008年第3期318-324,共7页动物学研究（英文）

基　　金：国家自然科学基金资助项目(C03020505)

摘　　要：灵长类动物的ABO血型抗原都表达在组织器官内,而不是在红细胞上,这给灵长类动物血型的鉴定带来很大的困难。为找到更加简捷、准确鉴定灵长类动物类人ABO血型的方法,采用近年来临床上广泛应用的卡式微柱凝胶正、反定型法对34只猕猴和16只食蟹猴的血型进行了鉴定,并与肾组织免疫组化法的检测结果进行比较。结果显示:卡式微柱凝胶正定型法的检测结果中无一例为阳性结果;血浆中的纤维蛋白原和人-猴种属间非特异性抗体都会对卡式微柱凝胶反定型法的部分检测结果产生干扰;采用经正常人O型红细胞吸附处理后的清亮血清,卡式微柱凝胶反定型法的检测结果明确,与免疫组化法判定结果一致。由此得出:卡式微柱凝胶反定型法可以用于灵长类动物血型的鉴定,其主要干扰因素为血浆内的纤维蛋白原和人-猴种属间非特异性抗体,在采用清亮血清及经正常人O型红细胞吸附处理后能消除其干扰。Monkeys do not express ABO antigens on red blood cells （RBCs）, but on tissues, which is an obstacle in ABO typing. In this study we describe a simple and efficient method to type monkeys blood. Rhesus monkeys （n=34） and cynomolgus monkeys （n=16） were typed by the direct and reverse gel system, which has been widely used in clinics in recent years. Based on the results of immunohistochemical staining, we evaluated the feasibility and the interference factors of the gel system to type monkeys. The results revealed that the direct gel system didn＇t have a positive report, the fibrinogen and non-specific anti-human antibodies in monkey blood interfered with the reverse gel system in some samples. In accordance with the results of immunohistochemical staining, using clear sera, which were pre-absorbed on normal human type O RBCs, the reverse gel system gave an accurate blood type determination for monkeys. We concluded that the reverse gel system could be used for ABO typing of monkeys, and the fibrinogen and non-specific anti-human antibodies were the major interference factors, which could be eliminated by using clear and pre-absorbed sera.

关 键 词：卡式微柱凝胶法 ABO血型 猴 

分 类 号：R446.6[医药卫生—诊断学]