注射法小鼠皮下子宫内膜异位症模型及组织学观察  

Establishment and Histological Observation of a New Mouse Model of Subcutaneous Endometriosis

作　　者：马丽[1] 谢淑武[2] 朱焰[2] 卢颖莹[1] 曹霖[2] 

机构地区：[1]复旦大学,上海200032 [2]上海市计划生育科学研究所

出　　处：《中国实验动物学报》2008年第3期192-195,I0005,共5页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的建立ICR小鼠皮下子宫内膜异位症新的模型及组织学观察。方法以同系小鼠作为供体,取子宫内膜分别采用皮下注射法和皮下植入法对2组共24只小鼠行皮下造模手术。3周后测量皮下种植结节大小,并处死半数造模成功小鼠,取子宫和异位结节行HE染色组织学研究。6周后,处死剩余小鼠,并再次测量皮下结节,取子宫和异位结节行HE染色组织学研究。结果皮下植入法和皮下注射法成功率分别为33.33%和83.33%(P<0.01);生长良好的囊肿囊内壁有内膜上皮细胞生长,有与正位子宫内膜接近同步的发情周期改变;病灶随移植时间的推延先逐渐增大之后又有缩小趋势(P>0.05)。结论将ICR小鼠子宫内膜注射到同系小鼠腹部皮下形成子宫内膜异位病理模型,成功率高,且简单可行,异位内膜随动情周期有周期性改变,生理特性与正位子宫内膜基本相同,应用于子宫内膜异位症疾病和药物研究既经济又稳定可靠。Objective To establish a ICR mouse model of subcutaneous endometriosis and study its histological features. Methods Uterine samples to be inoculated were obtained from syngenic mice used as donor mice. Endometriosis was surgically induced in 24 ICR mice （2 groups） by placing or injecting into subcutaneous tissue, respectively. Three weeks after implantation the ectopic endometrium was measured and a half of the ectopic endometrium and uterus was taken for histological examination. Six weeks after implantation the rest mice were killed and the ectopic endometrium was measured and samples were taken for microscopic examination. Results There were 33.33% and 83.33% of the mice in the implantation and injection groups developed endometriosis, respectively （P 〈 0.01 ）. The inner wall of the cyst had endometrial epithelial cells, and it altered consistently with the menstrual cycle of the normal endometrium. The cyst enlarged gradually at first and had a contraction tendency afterwards （P 〉 0.05 ）. Conclusion A subcutaneous model of endometriosis in ICR mice has been established by injecting the endometrium from syngenic donor mice into subcutaneous tissue. The ectopic endometrium changes correspondingly to the reproductive cycle, and exerts physiological functions alike the normal endometrium. It is a convenient and successful method to generate an animal model for researches on endometriosis and drug development.

关 键 词：皮下疾病模型 ICR小鼠 子宫内膜异位症/病理学 

分 类 号：R711.71[医药卫生—妇产科学]