核酸及其衍生物的代谢加工运输相关基因在大鼠肝再生中表达模式的分析  

ANALYSIS OF EXPRESSION PATTERNS OF NUCLEIC ACID AND THEIR DERIVATIVE METABOLISM,PROCESSING AND TRANSPORT-RELATED GENES DURING RAT LIVER REGENERATION

作　　者：胡珂[1] 徐存拴 

机构地区：[1]河南省-科技部共建细胞分化调控重点实验室,新乡453007 [2]河南师范大学生命科学学院

出　　处：《解剖学报》2008年第3期329-334,共6页Acta Anatomica Sinica

基　　金：国家973计划前期研究专项(2006CB708506)资助

摘　　要：目的在基因转录水平了解核苷酸及其衍生物、DNA及RNA代谢、加工、运输相关基因在大鼠肝再生（LR）中的表达变化。方法用搜索网站资料和查阅相关论文等方法获得核苷酸及其衍生物、DNA及RNA代谢、加工、运输相关基因,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用手术组和假手术组比较的方法确定肝再生相关基因。结果初步证实,上述基因中有240个基因与大鼠肝再生相关。肝再生早期[部分肝切除（PH）后0.5～4h]、前期（PH后6～12h）、中期（PH后16～66h）、后期（PH后72～168h）等4个时期起始表达的基因数分别为65、14、150和11,基因的总表达次数为133、87、627和169,它们的表达模式分别为6、5、22和9种,共表达上调768次,下调248次。肝再生前期和中期mRNA降解增强;前期、中期和后期DNA重组、修饰、转录及mRNA加工和运输增强;几乎整个肝再生中核苷酸及其衍生物代谢、DNA复制、包装和分解活动增强。结论肝再生相关基因主要在肝再生早期起始表达,在不同时期发挥作用。Objective To study the expression changes and patterns of nucleic acid and their derivative metabolism, processing and transport-related genes in rat liver regeneration （LR） at transcriptional level. Methods The related genes were obtained by online data collection and literature review, and gene expression patterns in rat regenerated liver were checked by Rat Genome 230 2.0 array. LR-related genes were identified through the comparison of discrepancies in gene expression between partial hepatectomy （PH） and sham operation （SO） groups. Results 240 genes were determined to be related with liver regeneration. The number of initially expressive genes in the forepart of liver regeneration （0.5-4 hours after PH）, prophase （6-12 hours after PH）, metaphase （16-66 hours after PH）, anaphase （72-168 hours after PH） were 65, 14, 150, 11 respectively and the total expression numbers were 133, 87, 627, 169 respeitirely. There were 6, 5, 22 and 9 expression patterns. Frequencies of up-regulation and down-regulation were 768 and 248. mRNA catabolism in prophase and metaphase, DNA recombination, modification and transcript, mRNA processing and transport in prophase, metaphase and anaphase, and nucleotides and their derivatives metabolism, as well as DNA replication, packaging and catabolism during the whole LR, were enhanced. Conclusion LR-related genes were mainly triggered in the forepart of liver regeneration, and funclioned at different phases.

关 键 词：部分肝切除 核酸 衍生物 肝再生相关基因 基因组2302.0芯片 大鼠 

分 类 号：R333.6[医药卫生—人体生理学]