神经元核抗原在大鼠中枢神经系统神经元的不同表达  

DIFFERENT EXPRESSIONS OF NEURONAL NUCLEI IN THE NEURONS OF RAT CENTRAL NERVOUS SYSTEM

作　　者：王新红[1] 段丽[1] 曹荣[1] 江山[1] 赵钢[2] 饶志仁[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032 [2]第四军医大学西京医院神经内科,西安710032

出　　处：《解剖学报》2008年第3期381-385,共5页Acta Anatomica Sinica

基　　金：全军“十五”科研基金重点课题(01Z082);“十一五”课题(06MA234)资助

摘　　要：目的观察正常大鼠中枢神经系统(CNS)内神经元核抗原(NeuN)的表达。方法正常成年SD大鼠5只,常规固定,恒冷箱切片,免疫组织化学、免疫荧光染色、尼氏染色。结果NeuN在成年大鼠CNS神经元有4种表达:大部分神经元(大脑皮质、基底核、脊髓背角等)有明显表达;部分神经元为中等表达(三叉神经脊束核、下丘脑室旁核等);部分神经元为弱表达(孤束核、蓝斑等);而有些核团神经元缺乏NeuN的表达(视上核、弓状核、迷走神经背核等)。结论中枢神经系统不同区域或核团的神经元NeuN的表达程度不同,有的区域或核团神经元缺乏NeuN表达。Objective The aim of this study was to observe the expression of NeuN in the central nervous system （CNS） of adult rats. Methods Normal adult SD rats were perfused and cut into sections on a cryostat. Immunohistochemistry, immunofluorescent and Nissl staining technique were used. Results Four types of NeuN expression in the neurons of CNS were observed： the neurons in most areas throughout the CNS （such as the cerebral cortex, basic ganglia and spinal cord dorsal horn, etc） were found with strong NeuN signals; the neurons in some areas （such as the spinal nucleus of trigemina nerve and the hypothalamic paraventricuar nucleus, etc） revealed middlling signals; the neurons in other nuclei indicated wealer NeuN signals （such as the solitary tract nucleus and the locus coeruleus, etc）; some areas （such as the supraoptic nucleus, the arcuate nucleus and the dorsal nucleus of vagus nerve, etc ） appeared devoid of immunoreactivity for NeuN. Conclusion The present results indicate that expressions of NeuN in all the neurons of CNS of adult rats were different, and NeuN even didn＇t express in some areas.

关 键 词：神经元核抗原 中枢神经系统 免疫组织化学 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]