大鼠肝促性腺激素释放激素受体的定位研究  

STUDIES ON LOCALIZATION OF GONADOTROPIN-RELEASING HORMONE RECEPTORS IN RAT LIVER

作　　者：谢菲[1] 姬秋和[1] 黄威权[1] 王艳霞[1] 

机构地区：[1]第四军医大学西京医院内分泌代谢科,西安710032

出　　处：《解剖学报》2008年第3期433-435,共3页Acta Anatomica Sinica

摘　　要：目的探讨大鼠肝是否表达促性腺激素释放激素(GnRH)受体,为GnRH能否参与肝代谢功能的调节提供形态学依据。方法采用兔抗GnRH的抗独特型抗体的免疫组织化学SABC法及地高辛标记探针的原位分子杂交法,对5例大鼠肝GnRH受体表达进行研究。结果大鼠肝细胞既呈GnRH受体阳性又有GnRH受体mRNA杂交信号。GnRH受体阳性物质和GnRH受体mRNA杂交信号物质均分布在大鼠肝细胞的细胞质,细胞核未检测到GnRH受体阳性物质及GnRH受体mRNA杂交信号。不同部位的肝细胞其GnRH受体免疫反应强度和GnRH受体mRNA杂交信号强度存在差别。结论大鼠肝细胞能表达GnRH受体,不同部位的肝细胞对GnRH受体表达水平存在差异。Objective To detect the existence of gonadotropin-releasing hormone receptors （GnRH-R） in rat liver, and to supply morphological evidence for studying the functional significance of the GnRH in hepatocyte. Methods Immunohistochemical SABC method and in situ hybridization with a digoxigenin labeled probe were used to study the localization of GnRH-R in the livers of five rats. Results The rat hepatocytes were found with the GnRH-R immunoreactivity and GnRH-R mRNA hybridization signals. The GnRH-R immunoreactive substance was distributed in the cytoplasm of all positive cells, with immuno-negative nuclei. The GnRH-R mRNA hybridization signals were also detected in the cytoplasm of all positive cells but not in the nuclei. Hepatocytes in different parts of liver lobules showed different intensity of GnRH-R immunoreactivity and GnRH-R mRNA hybridization signals. Conclusion The rat hepatocyte may express GnRH-R. The hepatocytes in different parts in liver lobules exhibit different levels of GnRH-R expression.

关 键 词：促性腺激素释放激素受体 肝 免疫组织化学 原位杂交 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]