小鼠肺钩虫感染基因表达谱的生物信息学分析—种系效应  

Bioinformatics Analysis of Gene Expression Pattern of Mice Lung-Stage Helminth Infection-Strain-Effect

作　　者：刘华[1] 马文丽[1] 丁大鹏[1] 柯杰兵[2] 郑文岭[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515 [2]中国人民解放军军事体育进修学院,广州510500

出　　处：《热带医学杂志》2008年第5期423-425,共3页Journal of Tropical Medicine

基　　金：广东省重点实验室资助项目(No.960327)

摘　　要：目的从基因水平探索肌萎缩侧索硬化(ALS)其发病机制并发现治疗ALS的新靶点。方法通过GeneSifter软件对野生型(WT)和重度联合免疫缺陷型(SCID)小鼠肺组织钩虫感染的微阵列表达谱进行分析,使用two-way ANOVA的统计学方法对数据聚类,并结合Gene Ontology和KEGG(Kyoto Encyclopedia of Genesand Genomes)生物学通路,深入分析具有种系效应的517个差异基因(P<0.001)。结果显示ALS的信号转导通路在两组小鼠间差异具有统计学意义(Z>2),与Th2反应相关的多个基因对ALS的代谢异常具有保护作用。结论筛选出的3个特异基因,可作为治疗肌萎缩侧索硬化的新候选基因。Objective To investigate the molecular pathogenesis of amyotrophic lateral sclerosis （ALS） at genetic level for the development of potential new drug targets. Methods Two-way ANOVA, Gene Ontology and KEGG methods were used for the analysis of gene expression pattern of Lung-Stage Helminth Infection in wild type and SCID mice with GeneSifier software. Further investigation was performed on 517 genes for strain-effect （P 〈 0.001 ）. Results The pathway of amyotrophic lateral sclerosis （ALS） was different significantly between the two strains of mice（Z 〉 2）. Several genes relevant with Th2 responses had a positive effect on the abnormal metabolic process of ALS. Conclusion 3 novel genes were identified as candidates that may have therapeutic benefits for amyotrophic lateral sclerosis.

关 键 词：GeneSifier软件 基因表达谱 肌萎缩侧索硬化 

分 类 号：R318.04[医药卫生—生物医学工程]