反复自然流产夫妇HLA-Cw等位基因多态性分析  

Analysis of HLA-Cw Alleles in Couples with Recurrent Spontaneous Abortion

作　　者：许成岩[1] 王珊[1] 石玉华[1] 陈子江[1] 焦玉莲[2] 赵跃然[1] 

机构地区：[1]山东大学附属省立医院生殖医学中心,济南250021 [2]山东大学附属省立医院中心实验室,济南250021

出　　处：《生殖与避孕》2008年第5期273-277,共5页Reproduction and Contraception

摘　　要：目的:探讨免疫遗传因素在反复自然流产(recurrent spontaneous abortion,RSA)发病中的作用。方法:采用序列特异性引物聚合酶链反应(PCR-SSP)法,分析80对RSA患者夫妇和75对正常夫妇HLA-Cw等位基因的多态性。结果:病例组和对照组间Cw01-08各个等位基因阳性率均无统计学差异,但RSA组中夫妇双方均检测到Cw第Ⅱ组等位基因(Cw分子α重链上第80位为赖氨酸)者显著少于对照组,而RSA组中夫妇双方均未检测到Cw第Ⅱ组等位基因者显著多于对照因缺失可能与早期反复自然流产的发病有关。组,差异有统计学意义(P=0.013)。Objective： To investigate the effect of immunal genetic factors on the pathogenesis of recurrent spontaneous abortion （RSA）, Methods： Eighty childless couples with three or more unexplained early abortions and 75 healthy couples as control subjects were genotyped for HLA-Cw alleles using PCR-SSP. Results： When the frequencies of HLA-Cw alleles were compared between patients and controls no detectable differences for any alleles could be observed. But from a cohort of husband and wife couples, the couple with a decreased group II HLA-Cw allele （HLA-Cw molecules containing Lys80） had a tendency to fall into the RSA group, and the couple with more group Ⅱ HLA-Cw alleles had a tendency to fall into control group （P=0.013）. Conclusion： Decreased HLA-Cw allele that HLA-Cw molecules containing Lys80 influences the susceptibility to RSA in the Chinese population.

关 键 词：基因 HLA-CW 反复自然流产 

分 类 号：R714.21[医药卫生—妇产科学]