肺癌细胞株中顺铂药物敏感性的相关基因  

DDP-sensitivity-related genes in 10 lung cancer cell lines

作　　者：李春红[1] 蔡莉[1] 陈雪松[1] 孟庆威[1] 隋广杰[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内科,150040

出　　处：《中华肿瘤杂志》2008年第6期418-421,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目（30772540）;黑龙江省科委重大攻关课题资助项目（GB05C401-07）

摘　　要：目的筛选小细胞肺癌（SCLC）和非小细胞肺癌（NSCLC）细胞株中与顺铂（DDP）药物敏感性相关的基因。方法采用二苯基溴化四氮唑蓝（MTT）比色分析法，测定4株SCLC细胞株和6株NSCLC细胞株对DDP的药物敏感性；应用cDNA微阵列技术检测肺癌细胞株中1291个药物敏感性相关基因的表达状态，并分析基因表达与DDP敏感性之间的相关性。结果Metallothionein、CathepsinB、TIMP1、TNF-R1、TGFβ-induced 68000、Cathepsin L、Galectin-1、Annexin 11、PAI-1、IGFBP4、UPAR、Jagged、CDl3、etlA．AR、EphA2（Eck）、APC、RhoC、Fibromodulin、GATA-6、HSC70共20个基因，在SCLC和NSCLC细胞株中均与DDP的药物敏感性呈负相关，只有Procoagula和MDM2与DDP的敏感性呈正相关。VHL、MMP-7、ElonginA、GSK-3B、SLC、Galectin-3、Integrinβ5、Moesin、IKKβ和ETV1等10个基因，只在SCLC细胞株中与DDP的药物敏感性呈负相关，而AT2与DDP敏感性呈正相关。Clusterin、FG FR-2、Thrombospondin 1、HSP 32、Lactate dehydrogenase A、P300、Thymosinβ10、CD81、C／EBPγ和Rak等10个基因，只在NSCLC细胞株中与DDP的药物敏感性呈负相关，而只有CaMKK和TPA与DDP的敏感性呈正相关。结论与DDP药物敏感性相关的基因共有45个，其中共同表达于SCLC和NSCLC细胞株中的基因有22个，在SCLC细胞株中特异表达的基因有11个，在NSCLC细胞株中特异表达的基因有12个。Objective To search for genes related to cisplatin （DDP） sensitivity in small-cell lung cancer （SCLC） and non-small-cell lung cancer （NSCLC） cell lines. Methods The sensitivity of 4 SCLC lines and 6 NSCLC lines to DDP was evaluated by MTT assay. The expression of 1291 genes related to DDP- sensitivity in the 10 cell lines was measured by cDNA macroarray and the relationship between genes and DDP-sensitivity was analyzed. Results 20 genes were negatively related to DDP-sensitivity in the SCLC and NSCLC cell lines, including Metallothionein, Cathepsin B, TIMP1, TNF-R1, TGFβ-induced 68 000, Cathepsin L, Galectin-1, Annexin 11, PAI-1, IGFBP4, UPAR, Jagged, CD13, α1 A-AR, EphA2 （Eck）, APC, RhoC, Fibromodulin, GATA-45 and HSC 70, while only procoagula and MDM2 were positively related to DDP-sensitivity in the SCLC and NSCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the SCLC cell lines, including VHL,MMP-7, Elongin A, GSK-313, SLC, Galectin-3, integrinβ5, moesin, IKKβ, and ETV 1, while only AT2 was positively related to DDP-sensitivity in the SCLC cell lines. 10 genes were negatively related to DDP-sensitivity in the NSCLC cell lines, including Clusterin, FG FR-2, Thrombospondin 1, HSP 32, Lactate dehydrogenase A, P300, Thymosinβ10, CDS1, C/EBPγ, Rak, while only CaMKK and TPA were positively related to DDP-sensitivity in the NSCLC cell lines. Conclusion There were 45 genes related to DDP-sensitivity in 10 lung cancer cell lines. There were 22 co-expressed genes in both SCLC and NSCLC cell lines, and only 11 and 12 genes expressed in the SCLC and NSCLC cell lines, respectively.

关 键 词：肺肿瘤 顺铂 药物敏感性相关基因 

分 类 号：R686[医药卫生—骨科学]