副溶血性弧菌的显色培养基检测及耐药性分析  

Isolation Vibrio parahaemolyticus by vibrio chromogenic medimn and analyzing of drug resistance to clinical isolates

作　　者：夏旭芬[1] 鲍亚萍[1] 张伟敏[1] 

机构地区：[1]浙江省立同德医院检验科,杭州310012

出　　处：《国际流行病学传染病学杂志》2008年第3期154-155,160,共3页International Journal of Epidemiology and Infectious Disease

摘　　要：目的研究弧菌显色培养基对副溶血性弧菌（Vp）的筛选性能及Vp在临床中的检出率和抗菌谱。方法肠道病患者粪便样本经碱性蛋白胨水增菌后，接种于弧菌显色培养基上，对淡紫色菌落进行生化鉴定分析，得出弧菌显色培养基的选择性能；药敏实验采用K-B法。结果802份粪便样本经碱性蛋白胨水增菌和弧菌显色培养基培养后，193份样本中长出淡紫色菌落。附加试验和生化反应符合Vp标准的菌株为178株，检出率为22．2％，弧菌显色培养基鉴定Vp的阳性预测值为92．0％。药敏结果显示，Vp对氨苄西林耐药率达82．0％，对Ⅰ代头孢菌素（头孢噻吩、头孢唑林）的耐药率为22．5％，而对Ⅲ代头孢菌素、氨基糖苷类、Ⅲ代喹诺酮类及其他抗生素的耐药率〈5．0％。结论弧菌显色培养基对肠道门诊患者Vp的检出简便快速，准确度较高；需要时可选用第Ⅲ代喹诺酮类或头孢菌素、氨基糖苷类和哌拉西林等抗生素进行治疗。Objective To study the feasibility of vibrio chromogenic medium isolating Vibrio parahaemolyticus （Vp） and the clinical Vp isolation and resistance rate of antibiotics. Method After bacterial enrichment in alkaline peptone water, stool samples of patients were inoculated in vibrio colored medium, and Vp traditional biochemical tests were applied later to identify the purple colonies on the plate. The resistance to antibiotics was can＇ied out by K-B test. Results In 802 samples of .diarrhea-out patient, there were 193 samples growing purple colonies on vibrio colored medium, in which 178 strains were identified as Vp by traditional biochemical tests. The clinical isolating rate of Vp was 22.2% and the positive predictive value for Vp of the colored medium was 92.0%. The rates of resistance to ampicillin, cefalothin and eefazolin were 82.0%, 22.5% and 22.5% respectively. The rates of resistance to other drugs used in this study were all below 5.0%. Conclusions The vibrio colored medium on isolating Vp is fast, simple and has good accuracy. The third generation of quinolones and cephalosporin, and the aminoglycosides could he chosen to treat Vp infection when needed.

关 键 词：弧菌 副溶血性 药敏试验 弧菌显色培养基 

分 类 号：R446.5[医药卫生—诊断学]