抗大豆胞囊线虫3号生理小种(SCN3)核心种质代表性分析  

Representative Analysis of the Establishment of a Core Collection Focused on Race 3 of Soybean Cyst Nematode

作　　者：段玉玺[1] 周博[1] 陈立杰[1] 吴海燕[1] 

机构地区：[1]沈阳农业大学,中国北方线虫研究所,辽宁沈阳110161

出　　处：《大豆科学》2008年第3期366-372,共7页Soybean Science

基　　金：国家自然科学基金资助项目(30671399);科技部成果转化基金资助项目(05EFN212100059)

摘　　要：大豆胞囊线虫（soybean cyst nematode，SCN）病是大豆生产中最严重的病害，这种病害最好的防治措施就是培育抗性品种。然而，中国大多数大豆育成品种都不抗SCN3。为了有效的研究和利用中国的大豆抗胞囊线虫种质资源，将64份中国小黑豆品种（包括鉴别寄主）对现有的1、2、3、4、5、7、14号7个大豆胞囊线虫（Heterodera glycirtes）生理小种进行了抗性鉴定，应用SPSS统计系统软件将供试品种的抗病基因进行分类；同时调查了在田间根部感染SCN3的胞囊数量。结果表明：在相似距离为0．52时，将供试64个品种按含有抗感大豆胞囊线虫生理小种的抗性基因差异，可以划分为7大类。在田间连续5年（2003～2007）跟踪调查不同大豆根部侵染的胞囊量情况，平均胞囊量为0的有应县小黑豆等共18个品种；平均胞囊量为0．07的有哈尔滨小黑豆等共8个品种；平均胞囊量为0．13的有平顶山和茶豆；其它各品种的平均胞囊量均在0．13～6．00之间。综合上述试验结果以及各品种的农艺性状，选用等比例取样法构建了由16份种质资源组成的抗SCN3初选核心种质。抗SCN3初选核心种质的建立，为深入了解中国大豆抗性种质的遗传多样性、研究SCN3复杂抗性遗传变异规律和发掘新基因提供了合理的样本，同时将有助于加速抗病育种的进程，满足生产上的需要。Soybean cyst nematode （Heterodera glycines;SCN） is one of the most important pests affecting soybean production, and the main preventing and controlling methods depend on the application of resistant variety. However, most soybean cultivars in China have no resistance to SCN. Physiological races 1,2,3,4,5,7 and 14 have been identified by the Nematode Institute of Northern China, using 64 different small black soybeans （ including identification host）. We classified the resistance genes by SPSS. The result showed that the 64 resistant cultivars could be divided into 7 clusters based on the difference of resistant genes to SCN3 when analogous distance equaled to 0.52. In this test,we investigated the cyst number to race 3 of SCN in the field（ 2003-2007 ）. Eighteen resistant cultivars including Yinsxianxiaoheidou with the average cyst number（ACN） of 0, eight resistant cultivars including Harbinxiaoheidou with the ACN of 0.07 were identified. The ACN of Pingdingshan and Chadou was 0.13 ,and from 0.13 to 6 for other resistant cultivars. The primary core collection of 16 soy- bean germplasms resistant to SCN3 was constrcuted based on the screening results and agronomic traits. This primary core collection will provide a rational framework for undertaking diversity surveys ,using genetic variation for the investigation of complex traits and for the discovery of novel traits.

关 键 词：初选核心种质 大豆胞囊线虫3号生理小种(SCN3) 大豆 胞囊量 抗性 

分 类 号：S565.1[农业科学—作物学]