大豆疫霉菌检测鉴定方法  

Methods for Detection and Identification of Phytophthora sojae

作　　者：刘春来 李新民 杨明秀[2] 

机构地区：[1]黑龙江省农科院植物保护研究所,黑龙江哈尔滨150086 [2]东北农业大学农学院植保系,黑龙江哈尔滨150030

出　　处：《大豆科学》2008年第3期527-531,共5页Soybean Science

基　　金：国家“十五”科技攻关资助项目(2004BA520A16-0106)

摘　　要：大豆疫霉菌是一种较难分离和培养的病原菌,在国内,也是一种重要的植物检疫对象。因此,对大豆疫霉菌分离、鉴定方法的研究显得尤为重要。其传统的分离检测鉴定方法主要有病组织分离法、土壤诱集检测法等,近年来,随着生物技术的发展,血清学技术、同工酶技术及核酸技术等已应用到大豆疫霉菌的检测鉴定中。现综述这些方法在大豆疫霉菌检测鉴定中的应用现状,并对这些方法的应用进行评价。Phytophthora sojae, as an important plant quarantine pathogen in China,is difficult to isolate and culture. It is im- portant to studying on the methods of isolating and identifying Phytophthora sojae. The traditional isolation and detection methods include pathogen isolated from the infected tissue and soil baiting. With the development of biology technology, se- rology, isoenzymes and nucleic acid technology have been applied for detection of Phytophthora sojae in recent years. The applied condition of these methods in detection and identification of Phytophthora sojae were reviewed and evaluated.

关 键 词：大豆疫霉菌 病原菌 检测 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]