慢性γ线照射复合苯、甲苯和CO吸入对家兔某些生物效应的观察(论著)  

The biological effects induced by chronic γrays irradiation combined with benzene,toluene and carbon monoxide inhalation in rabbits.

作　　者：张鸿源[1] 王月兴[1] 王兰金[1] 潘燕[1] 范正平[1] 

机构地区：[1]海军医学研究所

出　　处：《中华航海医学杂志》1997年第4期204-208,共5页

摘　　要：目的：探索γ线和多种化学因素复合作用的生物效应规律。方法：按四因素二水平正交表Ｌ８（２７）设计，用家兔在密闭舱内进行８次试验。γ线二剂量水平分别为０．０３７５和０．００７５Ｇｙ／ｄ，苯、甲苯、一氧化碳（ＣＯ）的二浓度水平分别为１８２±３３和４０±１５ｍｇ／ｍ３、４０７±６８和９０±３０ｍｇ／ｍ３、２７８±８和９３±４ｍｇ／ｍ３。实验每天２小时，每周５天，持续８周。结果：（１）甲苯引起淋巴细胞明显减少，ＣＯ导致白细胞总数、血小板、血红蛋白明显减少，γ线和甲苯对血小板减少以及γ线和苯对血红蛋白降低，都呈现明显的交互作用。（２）γ线、苯和甲苯都能单独诱发淋巴细胞染色体畸变、微核、姐妹染色单体互换（ＳＣＥ）以及骨髓细胞染色体畸变，但未发现ＣＯ对诱发染色体畸变、微核和ＳＣＥ有明显影响。（３）γ线和苯诱发淋巴细胞染色体畸变和微核，γ线和甲苯以及苯和甲苯诱发淋巴细胞染色体畸变，都呈现明显的交互作用。（４）在８次复合试验的细胞遗传效应中，各指标均以四因素为高水平组合的①号试验最高，但即使是四因素皆为最低水平的⑧号试验，各指标亦均高于对照组，且除ＳＣＥ外，染色体畸变和微核都有统计学差异。⑤γ线、甲苯和ＣＯ均可引起睾丸重量指数?Aim:To ascertain the law of biological effects induced by γrays irradiation combined with benzene,toluene and carbon monoxide(CO) inhalation.Methods:8 tests were conducted according to four factors two doselevels orthogonal layout program.90 healthy male rabbits were equally divided into 8 test groups and a control group (10 in each group).The test animals were exposed to γrays combined with benzene,toluene and CO vapour for 2 h a day and 5 days per week for 8 weeks.The two doselevels γrays irradiation were 00375 and 00075 Gy/d,and the two concentration levels of benzene,toluene and CO were 182±33 and 40±15 mg/m 3,407±68 and 90±30 mg/m 3,and 278±8 and 93±4 mg/m 3 respectively.Results:(1) Toluene caused decrease in lymphocytes and CO caused decrease in total amount of leucocytes,thrombocytes and hemoglobin.The combinations of γrays with toluene caused decrease in thrombocytes and with benzene caused decrease in hemoglobin.(2) γrays,benzene and toluene were shown all induced chromosome aberrations,SCEs and micronuclei of lymphocytes and chromosome aberrations of bone marrow cells;but CO showed no effect.(3) γrays and benzene induced chromosome aberrations and micronuclei of lymphocytes;γrays and toluene induced chromosome aberrations of lymphocytes;benzene and toluene induced chromosome aberrations of lymphoytes;and all of them showed significant interaction. (4) In the 8 combined tests of cytogenetic effects,the test ① having high level combination of 4 factors had the highest measurements,but even the test ⑧ with lowest level of 4 factors were having higher measurements than that of the control.Furthermore,with the exception of SCE,the yields of chromosome aberrations and micronuclei were statistically significant also.(5) γrays,toluene and CO could cause obvious decrease of testis weight index and increase of aberrant sperm. Moreover,there were significant combined effects of γrays and toluene, and they could markedly reduce the number of sperm.(6) Patho

关 键 词：毒理 Γ线 苯 甲苯 生物效应 一氧化碳 

分 类 号：R994.6[医药卫生—毒理学] R818.03[医药卫生—药学]