毁灭柱孢抗生物质产生的摇瓶培养条件研究  

Submerged culture conditions for antifungal substances production by Cylindrocarpon destructans

作　　者：秦刚[1] 曾大兴[2] 姜子德[1] 

机构地区：[1]华南农业大学生物防治教育部工程研究中心,广东广州510642 [2]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《仲恺农业技术学院学报》2008年第1期10-13,共4页Journal of Zhongkai Agrotechnical College

基　　金：广东省自然科学基金团队项目(E05202480)资助

摘　　要：通过液体振荡培养,就不同培养基及初始pH、培养时间和培养温度等培养条件对毁灭柱孢(Cylindro-carpon destructans)D-5菌株产生抗生物质的影响进行了研究.结果表明,马铃薯蔗糖培养基是D-5菌株产生抗生物质最好的培养基;培养基初始pH6、培养温度22℃、摇床转速250 r/min、250 mL三角瓶摇甁装量80 mL为D-5菌株的最适振荡培养条件;抗生物质的产生随培养时间的延长而增加,4 d后达到最高.The effects of various media and culture conditions, including initial pH, fermentation duration, culture temperature, on the production of antifungal substances by Cylindrocarpon destructans were investigated in shake-flask cultures. The results showed that potato sucrose broth was the best medium for the production of antifungal substances, and that pH 6, 22 ℃, 250 r/min and 80 mL medium in a 200 mL- flask were found to be the optimal conditions for submerged culture. The production of antifungal substances was higher with longer culture time, and peaked after 4 days of cultivation.

关 键 词：毁灭柱孢(Cylindrocarpon destructans) 抗生物质 生物防治 

分 类 号：Q93-335[生物学—微生物学] S476.8[农业科学—农业昆虫与害虫防治]