知母皂甙元对原代培养的神经细胞M受体密度和代谢动力学的影响  

The effects of ZMS on the M cholinergic receptor density and its metabolic kinetic parameters in primary clutured neonatal rat brain cells

作　　者：范国煌[1] 易宁育[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室

出　　处：《中国中医基础医学杂志》1997年第6期15-17,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金

摘　　要：本文目的是用原代培养的神经细胞探讨知母皂甙元（ＺＭＳ）对神经细胞Ｍ受体的调节作用。主要方法是：用原代培养的神经细胞，用Ｍ受体非选择性拮抗剂３Ｈ－ＱＮＢ结合分析法测定Ｍ受体密度，并以Ｍ受体不可逆阻断剂苯甲基偶酰基胆碱氮芥（ＢＣＭ）为工具，测定Ｍ受体代谢动力学参数。主要结果：向不同培养天数（９～１５天）的神经细胞加入ＺＭＳ（０．１ｍｍｏｌＬ－１），作用４８小时后对Ｍ受体密度均有显著的上调作用（Ｐ＜０．０１）。各种浓度的ＺＭＳ（０．１～１００ｕｍｏｌＬ－１）对３Ｈ－ＱＮＢ的结合均无明显抑制作用。用（０．１ｍｍｏｌＬ－１）的ＺＭＳ显著升高Ｍ受体的合成速率（Ｐ＜０．０１），而对降解速率常数无影响（Ｐ＞０．０５）。结论：ＺＭＳ能使培养９～１５天的原代配养的大鼠神经细胞的Ｍ受体密度上调，这种作用和激动剂或拮抗剂不同，并非ＺＭＳ直接作用于Ｍ受体结合位点所引起，而是由于ＺＭＳ能促进Ｍ受体的生成。离体培养的环境相对衡定，可以排除神经体液的间接作用。Aim: To study the regulatory effects of ZMS, a Sapogenin from Zhimu (Rhizoma Anemarrhenae) on the neuronal muscarinic cholinergic receptors of primary culture of neonatal rat brain. Method:\ muscarinic antagonist 3H QNB was used to determine the receptor and the metabolic kinetic parameters were obtained by using the irreversible muscarinic blockade Benzilyl choline mustrad (BCM). Results:\ After being added to the culture medium for 48 hours, ZMS (0.1 mmol·L-1) significantly upregulated muscarinic receptor densities in different culture period ( P <0.01). No marked inhibition of 3H QNB binding was found when the range of ZMS was from 0.1￣100 umol·L-1. ZMS significantly elevated the production rate of neuronal muscarinic receptors ( P<0.01 ), with no influence on dissociation rate constant ( P>0.05 ). Conclusion:\ ZMS can significantly evelate the density of M cholinergic recptors in primary cultured rat brain cells. Such an action is not due to the direct binding of ZMS to the cells like agonists or antagonists. Instead, it is due to an stimulatory action of ZMS on the synthesis of M receptors

关 键 词：中药 知母皂甙元 原代培养 神经细胞 M受体 

分 类 号：R282.710.5[医药卫生—中药学]